Increasing evidence suggests that catalase may be an important enzyme in the metabolism of lipids, especially cholesterol (Caravaca & May, 1964; Caravaca & Dimond, 1967; Cuadrado & Bricker, 1973), and may function with other peroxisomal enzymes in steroidogenesis (Black & Bogart, 1973). Catalase-bearing peroxisomes have been described in Leydig cells of the rodent testis (Reddy & Svoboda, 1972). Maturation of the testis is known to be associated with changes in testicular lipids (Davis, Bridges & Coniglio, 1966; Oshima & Carpenter, 1968; Ichihara, 1969) and testosterone (Lipsett & Tullner, 1965; Warren, Haltmeyer & Eik-Nes, 1973), but there has been no information on testicular catalase activity changes with age. The present report describes the level of catalase in the prepubertal and mature rabbit testis, and correlates the findings with morphological changes during testicular development.
In order to demonstrate the effect to the acute administration of ethanol and chlorpromazine (CPZ) on bile flow and transhepatic transport of horseradish peroxidase (HRP) into bile, male rats were administered either 5 gm per kg ethanol intragastrically (E-rats) or 3 mg per kg CPZ intraperitoneally (CPZ rats). Control rats (C-rats) received saline. Two hours after ethanol feeding or 90 min after CPZ injection HRP was injected into the portal vein, and bile samples were collected at 10-min intervals for 2 hr. Tissue samples were removed at 1, 10, 60, and 120 min to study HRP transport using electron microscopic cytochemical localization. Bile flow was reduced (p less than 0.001) both in E- and CPZ-rats compared to C-rats. In E-rats HRP secretion was significantly decreased at 30 and 40 min post-HRP injection (p less than 0.05) and the peak rate of HRP secretion was delayed by 10 min compared to C-rats. Uptake and transhepatic transport of HRP were similar to controls. These results suggest that bile secretion and flow were impaired by ethanol. CPZ inhibited secretion of HRP significantly (p less than 0.001) during the first hr after HRP injection and by 25% after 2 hr. In CPZ rats studied cytochemically HRP reaction product decreased in the cytoplasm of hepatocytes 10 min after HRP injection (p less than 0.01). These findings suggest that acute CPZ administration caused an inhibition of the uptake of HRP as well as secretion and bile flow.
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