We determined the taxonomic composition of the bacterioplankton of the epipelagic zone of the Atlantic Ocean along a latitudinal transect (51°S–47°N) using Illumina sequencing of the V5-V6 region of the 16S rRNA gene and inferred co-occurrence networks. Bacterioplankon community composition was distinct for Longhurstian provinces and water depth. Free-living microbial communities (between 0.22 and 3 μm) were dominated by highly abundant and ubiquitous taxa with streamlined genomes (e.g., SAR11, SAR86, OM1, Prochlorococcus) and could clearly be separated from particle-associated communities which were dominated by Bacteroidetes, Planktomycetes, Verrucomicrobia, and Roseobacters. From a total of 369 different communities we then inferred co-occurrence networks for each size fraction and depth layer of the plankton between bacteria and between bacteria and phototrophic micro-eukaryotes. The inferred networks showed a reduction of edges in the deepest layer of the photic zone. Networks comprised of free-living bacteria had a larger amount of connections per OTU when compared to the particle associated communities throughout the water column. Negative correlations accounted for roughly one third of the total edges in the free-living communities at all depths, while they decreased with depth in the particle associated communities where they amounted for roughly 10% of the total in the last part of the epipelagic zone. Co-occurrence networks of bacteria with phototrophic micro-eukaryotes were not taxon-specific, and dominated by mutual exclusion (~60%). The data show a high degree of specialization to micro-environments in the water column and highlight the importance of interdependencies particularly between free-living bacteria in the upper layers of the epipelagic zone.

This study tests the hypothesis that horizontal density gradients have the potential to significantly contribute to the accumulation of suspended particulate matter (SPM) in the Wadden Sea. It is shown by means of long-term observations at various positions in the Wadden Sea of the German Bight that the water in the inner regions of the Wadden Sea is typically about 0.5-1.0 kg m Ϫ3 less dense than the North Sea water. During winter this occurs mostly because of freshwater runoff and net precipitation; during summer it occurs mostly because of differential heating. It is demonstrated with idealized one-dimensional water column model simulations that the interaction of such small horizontal density gradients with tidal currents generates net onshore SPM fluxes. Major mechanisms for this are tidal straining, estuarine circulation, and tidal mixing asymmetries. Three-dimensional model simulations in a semienclosed Wadden Sea embayment with periodic tidal forcing show that SPM with sufficiently high settling velocity (w s ϭ 10 Ϫ3 m s Ϫ1) is accumulating in the Wadden Sea Bight because of density gradients. This is proven through a comparative model simulation in which the dynamic effects of the density gradients are switched off, with the consequence of no SPM accumulation. These numerical model results motivate future targeted field studies in different Wadden Sea regions with the aim to further support the hypothesis.

Nitrogen (N) loss rates were determined in permeable sediments of the Wadden Sea using a combination of stable N isotope incubation experiments and model simulation approaches during three seasons. Three different incubation methods that employed the isotope pairing technique were used: intact core incubations simulating either (1) diffusive or (2) advective transport conditions and (3)

of the original manuscript: Staneva, J.; Stanev, E.V.; Wolff, J.-O.; Baldewien, T.H.; Reuter, R.; Flemming, B.; Bartholomae, A.; Bolding, K.:Abstract This work deals with analysis of hydrographic observations and results of numerical simulations. The data base includes ADCP observations, continuous measurements on data stations and satellite data originating from the Medium Resolution Imaging Spectrometer (MERIS) onboard the ESA satellite ENVISAT with a spatial resolution of 300 m. Numerical simulations use nested models with horizontal resolutions ranging from 1 km in the German Bight to 200 m in the East Frisian Wadden Sea coupled with a suspended matter transport model. Modern satellite observations have now a comparable horizontal resolution with high-resolution numerical model of the entire area of the East Frisian Wadden Sea allowing to describe and validate new and so far unknown patterns of sediment distribution. The two data sets are consistent and reveal an oscillatory behavior of sediment pools to the north of the back-barrier basins and clear propagation patterns of tidally driven suspended particulate matter outflow into the North Sea. The good agreement between observations and simulations is convincing evidence that the model simulates the basic dynamics and sediment transport processes, which motivates its further use in hindcasting, as well as in the initial steps towards forecasting circulation and sediment dynamics in the coastal zone.

The Southern Ocean constitutes about 10% of the global oceans' volume and is characterized by high primary production. Particulate organic matter (POM) is exported from the photic zone to the deep ocean and sustains life of particle associated (PA) and free-living (FL) bacterial communities in the dark realm. Little is known about the composition and diversity of PA and FL bacterial communities below the photic zone and how they differ among various regions of the Southern Ocean. Therefore, we investigated the composition of small (3-8 lm) and large (> 8 lm) PA and FL (0.2-3 lm) bacterial communities between 500 m and 3600 m in the Bransfield Strait, Drake Passage, and the south Atlantic Ocean featuring also Southern Ocean water masses. PA bacterial communities had a higher OTU richness and evenness than FL ones. Taxonomic analysis revealed a different community composition between FL and PA bacteria. A large number of OTUs belonging to diverse phyla (Bacteroidetes, Planctomycetes, Betaproteobacteria, Deltaproteobacteria, and Verrucomicrobia) were significantly enriched on particles; in contrast very few bacterial lineages were FL specialists. Lifestyle (FL vs. PA) and region (Bransfield basin vs. other regions) strongly influenced bacterial communities. Depth explained only marginal fraction of the total variation ( 12%), suggesting that selective processes driven by depth have a smaller effect in the Southern Ocean when compared to life-style (25%) and region (31%). Overall these data indicate a strong influence of isolated water masses such as the basin of the Bransfield Strait on the composition of bacterial communities in the dark ocean.