Many transition metal complexes have unique physicochemical properties that can be efficiently exploited in medicinal chemistry for cancer treatment. Traditionally, double‐stranded DNA has been assumed to be the main binding target; however, recent studies have shown that nucleosomal DNA as well as proteins can act as dominant molecular binding partners. This has raised new questions about the molecular determinants that govern DNA versus protein binding selectivity, and has offered new ways to rationalize their biological activity and possible side effects. To address these questions, molecular simulations at an atomistic level of detail have been used to complement, support, and rationalize experimental data. Herein we review some relevant studies—focused on platinum and ruthenium compounds—to illustrate the power of state‐of‐the‐art molecular simulation techniques and to demonstrate how the interplay between molecular simulations and experiments can make important contributions to elucidating the target preferences of some promising transition metal anticancer agents. This contribution aims at providing relevant information that may help in the rational design of novel drug‐discovery strategies.
To find out whether the AGO-miRNA complex is more sensitive to the accessibility of a particular region inside the seed match, we analyze in detail the accessibility of a wide set of miRNA binding sites validated by PAR-CLIP and HITS-CLIP experiments. Our analysis reveals that nucleotides at the 39-end of bound seed matches are significantly more accessible than nucleotides at the 59-end as well as nucleotides at any positions in the unbound seed matches. We show that the accessibility of a single nucleotide at the 39-end is more effective than the accessibility of several nucleotides at the 59-end in discriminating between functional and nonfunctional binding sites. Analysis of mRNA and protein fold changes induced by miRNA overexpression demonstrates that genes with accessible nucleation regions at the 39-end are down-regulated more strongly than genes whose accessible nucleation regions are located elsewhere within the seed match. We also observed an increase in the precision of the miRNA target prediction algorithm PACMIT when accessibility toward the 39-end of the seed match was required. The pronounced sensitivity of the AGO-miRNA complex to the accessibility of the 39-end of the seed match suggests that, in most cases, nucleation occurs in this region. We show that this conclusion is consistent with previous experimental studies.
Understanding how small molecules interact with DNA is essential since it underlies a multitude of pathological conditions and therapeutic interventions. Many different intercalator compounds have been studied because of their activity as mutagens or drugs, but little is known regarding their interaction with nucleosomes, the protein-packaged form of DNA in cells. Here, using crystallographic methods and molecular dynamics simulations, we discovered that adducts formed by [(η(6) -THA)Ru(ethylenediamine)Cl][PF6 ] (THA=5,8,9,10-tetrahydroanthracene; RAED-THA-Cl[PF6 ]) in the nucleosome comprise a novel one-stranded intercalation and DNA distortion mode. Conversely, the THA group in fact remains solvent exposed and does not disrupt base stacking in RAED-THA adducts on B-form DNA. This newly observed DNA binding mode and topology dependence may actually be prevalent and should be considered when studying covalently binding intercalating compounds.
Targeting defined histone protein sites in chromatin is an emerging therapeutic approach that can potentially be enhanced by allosteric effects within the nucleosome.Here we characterized an ovel hetero-bimetallic compound with ad esign based on an ucleosomal allostery effect observed earlier for two unrelated drugs-the Ru II antimetastasis/antitumor RAPTA-T and the Au I anti-arthritic auranofin. The Ru II moiety binds specifically to two H2A glutamate residues on the nucleosome acidic patch, allosterically triggering ac ascade of structural changes that promote binding of the Au I moiety to selective histidine residues on H3, resulting in cross-linking sites that are over 35 distant. By tethering the H2A-H2B dimers to the H3-H4 tetramer,the hetero-bimetallic compound significantly increases stability of the nucleosome,i llustrating its utility as as ite-selective cross-linking agent.
Understanding hows mall molecules interact with DNAisessential since it underlies amultitude of pathological conditions and therapeutic interventions.M any different intercalator compounds have been studied because of their activity as mutagens or drugs,but little is knownregardingtheir interaction with nucleosomes,t he protein-packaged form of DNAi nc ells.H ere,u sing crystallographic methods and molecular dynamics simulations,w ed iscovered that adducts formed by [(h 6 -THA)Ru(ethylenediamine)Cl][PF 6 ]( THA = 5,8,9,10-tetrahydroanthracene; RAED-THA-Cl [PF 6 ]) in the nucleosome comprise an ovel one-stranded intercalation and DNAd istortion mode.C onversely,t he THA group in fact remains solvent exposed and does not disrupt base stacking in RAED-THA adducts on B-form DNA. This newly observed DNAbinding mode and topology dependence may actually be prevalent and should be considered when studying covalently binding intercalating compounds.
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