PURPOSE:To evaluate macro and microscopically the evolution of autotransplants of fragments of spleen different fragments in the greater omentum, after eight weeks of observation. METHODS:Twenty rats Wistar were used, males and adults, submitted to total splenectomy and divided in two groups. The group Iten animals with implant of spleen fragment (25% weight of spleen) in the omentum; and group II -ten animals with implant of spleen fragment (30% weight of spleen) in the omentum. It was analyzed macro and microscopically the evolution of the implant. RESULTS:It was observed adherences to the adjacent tissues and vascularization in all of the fragments transplanted. The group I and II presented white pulp with follicular formations and lymphoid tissue preserved, and the red pulp in cordon aspect. The group II presented white pulp more disorganized and red pulp hemorrhagic. The active macrophages were observed in the group I and II. CONCLUSION:The splenic autotransplantation of the group I showed better regeneration.Key words: Transplantation, Autologous. Spleen. Histology. Rats. RESUMO OBJETIVO:Avaliar macro e microscopicamente a evolução do autotransplante de diferentes fragmentos de baço no omento maior, após oito semanas de observação. MÉTODOS:Foram utilizados 20 ratos Wistar, machos e adultos, submetidos a esplenectomia total e distribuídos em dois grupos. O grupo I -dez animais com implante de fragmento com 25% do peso do baço no omento e o grupo II -dez animais com implante de fragmento com 30% do peso do baço no omento. Foram observados macro e microscopicamente a evolução dos implantes. RESULTADOS:Foi observada no fragmento transplantado aderência aos tecidos adjacentes e vascularização preservada. Os grupos I e II apresentaram polpa branca e vascularização preservada, polpa branca com formação folicular e tecido linfóide preservado, e a polpa vermelha com aspecto cordonal. O grupo II apresentou polpa branca mais desorganizada e polpa vermelha hemorrágica. Os macrófagos ativos foram observados nos grupos I e II. CONCLUSÃO:O autotransplante esplênico do grupo I mostrou melhor regeneração.Descritores: Transplante Autólogo. Baço. Histologia. Ratos. Histological aspects of autologous transplantation of different fragments of the spleen in rats

Purpose:To analyze the triple antimicrobial therapy in positive Helicobacter spp. dogs and to investigate recurrence. Methods: A total of 20 dogs underwent endoscopy followed by gastric biopsy using the rapid urease test and histopathology stained with Giemsa. Ten animals were treated with triple therapy recommended for humans and divided into control and experimental group. The control group was kept in isolation while the experimental group was placed in contact with positive animals during 60 days. Results: The prevalence of infection in animals in this experiment was 100%, and more frequent in the fundus and the gastric body. Therapy for 7 days using clarithromycin, amoxicillin and lansoprazole was effective in 100% of the animals. Recurrence of the infection in 80% of dogs in the experimental group, while the control group remained eradicated after 60 days. Conclusion: Crowded environments associated with close contact with dogs infected with helicobacter are a determinant for transmission of Helicobacter spp. between canines. Key works: Helicobacter infections. Endoscopy. Gastric Mucosa. Dogs. RESUMOObjetivo: Avaliar a eficácia da terapia tríplice em cães naturalmente infectados pelo Helicobacter spp. e investigar a recorrência da infecção pelo contato com animais infectados. Métodos: Foram utilizados 20 cães, submetidos à endoscopia digestiva alta seguida de biopsia gástrica usando teste rápido da urease e histopatologia corada pelo Giemsa. Dez animais foram tratados com terapia tríplice preconizada para humanos e divididos em grupo controle e experimental. O grupo controle foi mantido em isolamento enquanto que o grupo experimento foi colocado em contato com os animais positivos durante 60 dias. Resultados: A prevalência da infecção nos animais deste experimento foi de 100%, e mais frequente no fundo e corpo gástrico. A terapia durante 7 dias empregando claritromicina, amoxicilina e lansoprazol foi eficaz em 100% dos animais. Houve recorrência da infecção em 80% dos cães do grupo experimental, enquanto que o grupo controle manteve-se erradicado após 60 dias. Conclusão: Ambientes aglomerados associado a íntimo contato com cães infectados por helicobactérias é fator determinante para transmissão do Helicobacter spp. entre caninos. Descritores: Infecção por Helicobacter . Endoscopia. Mucosa Gástrica. Cães. Acta Cirúrgica Brasileira -Vol. 26 (2) 2011 -83Studies of distribution and recurrence of Helicobacter spp. gastric mucosa of dogs after triple therapy

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Mammary neoplasms are the most common tumors in female dogs. They are usually treated using solely surgical mastectomy-which is recognized as unsatisfactory in many cases. Given this, the benefits of chemotherapy in dogs with mammary cancer need to be further explored. Some drugs that can be used for treating canines with mammary tumors may be substrates of uptake and/or efflux transporters such as the ATP-binding cassette (ABC) transporters. Unfortunately, very little is known regarding the pathobiology of such proteins in canine tumors, including mammary cancer. Accordingly, this study was designed to characterize the expression of ABC transporters Pglycoprotein, MRP1, and MRP2 and their relation with clinicopathologic factors in order to allow a better understanding of their influence in canine mammary cancer. P-glycoprotein was expressed in tumors from 55.8% of patients, while MRP1 and MRP2 were expressed in 37.2% and 39.5% of tumors, respectively. P-glycoprotein expression showed to be related with regional lymph node spread (P = 0.0038), as well as with tumor grade (P = 0.0353) and with a shorter survival (P = 0.0245). MRP1 revealed a strong association with a higher histological grade (P < 0.0001) and overall survival (P = 0.0002). Additionally, MRP1 was determined as prognostic indicator independent of lymph node status using Cox proportional-hazards regression multivariate analysis (P * Corresponding author. B. S. Salgado et al. 78= 0.0216). No relations between MRP2 and clinicopathologic features were observed. We have found that P-glycoprotein and MRP1 are expressed in highly aggressive canine mammary tumors and are related with poor prognosis. Our results suggest that they may play a significant role in the course of canine mammary cancer progression and be promising candidate markers for a validation study on therapy outcome.