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A water soluble polysaccharide and its selective precipitated fractions (F1, F2, and F3) were isolated from hot water extract of fruiting bodies ofAgaricus blazeiMurril known asAgaricus brasiliensisto evaluate the analgesic activity of the materials in Swiss mice. The isolated material and its fractions were characterized by two-dimensional COSY and HMQC spectra asβ-(1→6)-α-(1→4)-glucan-bound-protein polysaccharide constituted mainly byβ-(1→6) glucan. The glucan-protein polysaccharide and its fractions were analyzed in relation to the protein, carbohydrate, and mineral elements contents. The analysis of the glucan-protein complex revealed a total carbohydrate content of 36.95% and protein conjugated content of 17.24%. The mineral elements found in larger proportion were K, Na, Ca, P, and Zn. The analgesic activity bioassay of theβ-(1→6)-α-(1→4)-glucan bound-protein polysaccharide and its fractions in Swiss mice indicated a significant effect.