In Vietnam, Celastrus hindsii Benth, a medicinal plant rich in secondary metabolites, has been used to alleviate distress caused by ulcers, tumors, and inflammation for generations. The occurrence of two phenotypes, Broad Leaf (BL) and Narrow Leaf (NL), has raised questions about the selection of appropriate varieties for conservation and crop improvement to enhance medicinal properties. This study examined molecular differences in C. hindsii by comparing protein profiles between the NL and BL types using 2D-PAGE and MS. Peptide sequences and proteins were identified by matching MS data against the MSPnr100 databases and verified using the MultiIdent tool on ExPASy and the Blast2GO software. Our results revealed notable variations in protein abundance between the NL and BL proteomes. Selected proteins were confidently identified from 12 protein spots, thereby highlighting the molecular variation between NL and BL proteomes. Upregulated proteins in BL were found to be associated with flavonoid and amino acid biosynthesis as well as nuclease metabolism, which probably attributed to the intraspecific variations. Several bioactive proteins identified in this study can have applications in cancer therapeutics. Therefore, the BL phenotype characterized by healthier external morphological features has higher levels of bioactive compounds and could be better suited for medicinal use.
This is the first study to establish a complete protocol for micropropagation of Rehmannia glutinosa from root segments. The study involved investigating the effect of plant growth regulators on in vitro shoot regeneration and rooting and identifying substrates supporting survival and growth performance of ex vitro seedlings. A Murashige and Skoog (MS) medium containing 30 g/L sucrose for shoot induction and 0.2 mg/L indole-3-acetic acid (IAA), 1 mg/L 6-benzylaminopurine (BAP), and 1 g/L polyvinylpyrrolidone (PVP) for shoot multiplication resulted in the highest number of shoots per explant and shoot height. Applying a medium containing 0.5 mg/L IAA and 1 g/L PVP yielded optimal rooting of the shoots grown in vitro. Compost enriched with microbial inoculants and perlite enhanced seedling growth better than that with organic biofertilizer-free substrates (soil and sand). We recommend the continuous production of micropropagated R. glutinosa seedlings from root segments under the aforementioned conditions as a possible propagation technique for crops of this species.
Background and Objective: Rehmannia glutinosa Libosch. is a well-known medicinal plant species thanks to its valuable catalpol content with proven pharmacological potentials. However, there was little evidence on improving the crop productivity with a high catalpol content and total root yield. The present study aimed to assess the influence of nitrogen (N), phosphorus (P) and potassium (K) fertilizers on the catalpol content of Rehmannia glutinosa roots sown under divergent plant spacing. Materials and Methods: The experiment was designed with two factors: Factor A includes five incremental levels of fertilizers (0, 60, 120, 180 and 240 kg haG 1 for N; 0, 40, 80, 120 and 160 kg haG 1 for P and K) and factor B includes three plant spacings of S1 = 15, S2 = 20 and S3 = 30 cm between rows and 20 cm between plants. High Performance Liquid Chromatography (HPLC) was employed to determine the catalpol content. Results: The results showed that a moderate level of N (180 kg haG 1 ) in combination with S3 might produce the highest root yield and catalpol content. Both P and K appeared better than N in catalyzing plants to gain higher yield and accumulate maximum catalpol content at the application rates of 120 kg haG 1 . Conclusion: The current study confirms the positive influence of N.P.K. and plant spacing on the total yield and catalpol content in roots of Chinese foxglove and practical information to promote the mass production for medicinal materials and pharmaceutical industries.
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