AbstractIntroductionAfrican swine fever (ASF) was officially reported in Vietnam in February 2019 and spread across the whole country, affecting all 63 provinces and cities.Material and MethodsIn this study, ASF virus (ASFV) VN/Pig/HaNam/2019 (VN/Pig/HN/19) strain was isolated in primary porcine alveolar macrophage (PAM) cells from a sample originating from an outbreak farm in Vietnam’s Red River Delta region. The isolate was characterised using the haemadsorption (HAD) test, real-time PCR, and sequencing. The activity of antimicrobial feed products was evaluated via a contaminated ASFV feed assay.ResultsPhylogenetic analysis of the viral p72 and EP402R genes placed VN/Pig/HN/19 in genotype II and serogroup 8 and related it closely to Eastern European and Chinese strains. Infectious titres of the virus propagated in primary PAMs were 106 HAD50/ml. Our study reports the activity against ASFV VN/Pig/HN/19 strain of antimicrobial Sal CURB RM E Liquid, F2 Dry and K2 Liquid. Our feed assay findings suggest that the antimicrobial RM E Liquid has a strong effect against ASFV replication. These results suggest that among the Sal CURB products, the antimicrobial RM E Liquid may have the most potential as a mitigant feed additive for ASFV infection. Therefore, further studies on the use of antimicrobial Sal CURB RM E Liquid in vivo are required.ConclusionsOur study demonstrates the threat of ASFV and emphasises the need to control and eradicate it in Vietnam by multiple measures.

Background: The first confirmed case of African swine fever (ASF) in Vietnam was reported officially in February 2019. To date, ASF virus (ASFV) have been detected in 63/63 provinces in Vietnam. Currently, real-time polymerase chain reaction (PCR) is considered to be a powerful tool for viral detection in field samples, including ASFV. However, some recent reports have suggested that mismatches in primer and probe binding regions may directly affect real-time PCR qualification, leading a false-negative result.Aim: This study aims to further examine a conflicting result obtained from two OIE recommended methods, conventional PCR and real-time PCR, for ASFV detection.Methods: Two ASF suspected pigs from different provinces in the north of Vietnam were selected for this study based on clinical signs and postmortem lesions. The different results obtained by OIE-recommended conventional PCR and real-time PCR were further analyzed by the Sanger sequencing method and virus isolation in combination with hemadsorption (HAD) test using porcine alveolar macrophages cells.Results: The results showed that when the primer sequence matched perfectly with the sequences of field isolates, a mutation in probe binding region was found, indicating that a single mismatch in the probe binding site may cause a false-negative result by real-time PCR in detecting ASFV in clinical samples in Vietnam. An agreement between conventional PCR, using PPA1/PPA2 primers and two golden standard methods, virus isolation in combination with HAD assay, and sequencing method was observed in this study.Conclusion: A single mismatch in the probe binding site caused a failse-negative result by realtime PCR method in field diagnosis of ASFV. The needs consideration when selecting the appropriate molecular diagnostic methods is based on the current databases of ASFV sequences,  particularly for epidemiological surveillance of ASF. Keywords: African swine fever, PCR, Pigs, Real-time PCR, Vietnam

This research is conducted to examine the process from the intention to purchase life insurance among people in two major cities of Hanoi and Ho Chi Minh City in Vietnam, based on the theoretical framework of theory of planned behavior. The study is conducted in Hanoi and Ho Chi Minh City in Vietnam through an online questionnaire of 358 people. Based on the data collected, the study used Cronbach's alpha, exploratory factor analysis, KMO and Bartlett's test and regression analysis by using SPSS software. The results show that life insurance buying behavior was influenced by purchase intention, attitudes, financial insight and product accessibility. Moreover, it was recognized higher weight of financial literacy and product accessibility in life insurance buying behavior. The research determined that financial knowledge could promote buying intention to actual purchase behavior in the life insurance area.

FORMOSAT-7/COSMIC-2 (FS7/C2) satellite was successfully launched in June 2019. The satellite provides about 5000 radio occultation (RO) soundings daily over the tropical and partial subtropical regions. Such plentiful RO soundings with high accuracy and vertical resolution could be used to improve model initial analysis for typhoon prediction. In this study, assimilation experiments with and without the RO data were conducted with the WRFDA hybrid system for the prediction of Typhoon Haishen (2020). The experimental results show a remarkable improvement in typhoon track prediction with RO data assimilation, especially when using a nonlocal refractivity operator. Results in cycling DA and forecast are analyzed and verified for the RO data impact. Diagnostics of potential vorticity (PV) tendency budget helps explain the typhoon translation induced by different physical processes in the budget. The typhoon translation is essentially dominated by horizontal PV advection, but the track deviation can increase due to the vertical PV advection with opposite effects in the absence of RO data. Sensitivity experiments for different model initial times, physics schemes, and RO observation amounts show positive RO data impacts on typhoon prediction, mainly contributed from FS7. Complementary, an improved forecast of Typhoon Hagupit (2020) is also illustrated for the RO data impact.

Monitoring the exchanges of CO2 and energy between terrestrial ecosystems and the atmosphere is one of the approaches with high accuracy to quantify the contribution of tropical rainforest to climate. The results of the study in Nam Cat Tien forest ecosystem from 2012 to 2014 illustrate that this ecosystem plays the role of carbon sinks during 11 months in a years (over 90%) and the average uptake reaches 455,8 gC/m2. In addition, about 74% of the net radiation (155,8 W/m2) that the ecosystem obtainsis transformed into latent heat and about 23% into sensible heat thanks to the ability to restrict surface flow, fix groundwater and remain moisture at the high level.