By use of a structure-based computational method for identification of structurally novel Janus kinase (JAK) inhibitors predicted to bind beyond the ATP binding site, a potent series of indazoles was identified as selective pan-JAK inhibitors with a type 1.5 binding mode. Optimization of the series for potency and increased duration of action commensurate with inhaled or topical delivery resulted in potent pan-JAK inhibitor 2 (PF-06263276), which was advanced into clinical studies.
In this whitepaper, the Manufacturing Technical Committee (MTC) of the Product Quality Research Institute has updated the 1997 Transdermal Drug Delivery Systems Scale-Up and Post Approval Change workshop report findings to add important new product development and control principles. Important topics reviewed include ICH harmonization, quality by design, process analytical technologies, product and process validation, improvements to control of critical excipients, and discussion of Food and Drug Administration’s Guidance on Residual Drug in Transdermal and Related Drug Delivery Systems as well as current thinking and trends on in vitro–in vivo correlation considerations for transdermal systems.
The
present study investigates the chemical composition governing
the physical properties of mono- and diglycerides (MDGs) at the microstructural
level, as a function of aging and lot-to-lot variability. The physical
structure of the MDG plays a vital role in ameliorating the emulsion
stability and is widely explored in diverse research horizons related
to the pharmaceutical, cosmetic, and food industries. In an effort
to understand the mechanism of emulsion stabilization, physical properties
were extensively evaluated in selective commercial lots to determine
if there is a correlation between the chemical composition of MDG
and physical properties. The solid state of the MDG samples with different
aging profiles was characterized using X-ray scattering, differential
scanning calorimetry, attenuated total reflection-Fourier transform
infrared spectroscopy, and NMR relaxometry. Moreover, the kinetic
aspect of solid-state transformation was also evaluated via treating
MDG samples with a heat–cool cycle. The chemical composition
of MDGs was quantified using a quantitative NMR (qNMR) method. Interestingly,
the X-ray scattering results demonstrated a change in the MDG polymorphic
form and an increase in the %β content as a function of aging.
The increase in the %β content led to the formation of rigid
crystal structures of MDG, as evident from the NMR relaxometry. Chemical
quantification of isomeric composition revealed chemical composition
change as a potentially critical factor responsible for the altered
physical structures of MDG with respect to aging and lot-to-lot variability.
The findings correlated the solid-state transformation with the change
in the chemical composition of the MDG as a combined effect of aging
and lot-to-lot variability. This work serves as a basis to better
understand the interdependency of the physicochemical properties of
MDG. Furthermore, the present work can also be used as guidance for
setting up the specifications of MDG, as per the required polymorphic
form for a multitude of applications.
Background. Transcription factor retinoic acid-related orphan receptor 2 (RORC2/ RORcT) mediates interleukin (IL)-17A and IL-17F expression. IL-17A plays a central role in the pathogenesis of several inflammatory disorders, including psoriasis. The RORC2 inhibitor PF-06763809 has been hypothesized to inhibit IL-17A production in T-helper 17 (Th17) cells, thereby reducing psoriasis symptoms. Aim. To assess the safety, tolerability and effect on skin infiltrate thickness of PF-06763809 in participants with mild/moderate chronic plaque psoriasis. Methods. This was a randomized, double-blind, first-inhuman study (trial registration: ClinicalTrials.gov NCT03469336). Participants received each of the following six treatments once daily for 18 days: three topical doses (2.3%, 0.8%, 0.23%) of PF-06763809, a vehicle and two active comparators (betamethasone and calcipotriol). Primary endpoints included change from baseline in psoriatic skin infiltrate thickness [echo-poor band (EPB) on ultrasonography] at Day 19, and safety. Change in psoriasis-associated gene expression (Day 19), evaluated by real-time reverse transcription PCR of skin biopsies, was an exploratory endpoint. Results. In total, 17 participants completed the study. Change from baseline in the EPB on Day 19 for all three doses of PF-06763809 was not significantly different from that of vehicle (P > 0.05). A significant reduction in EPB from baseline was observed with betamethasone on Day 19 relative to all other treatments (P < 0.0001). Treatment-related adverse events were mild/moderate. There were no significant differences in gene expression on Day 19 between vehicle and PF-06763809-treated skin lesions. Conclusion. Using a psoriasis plaque test design, PF-06763809 was found to be well tolerated with an acceptable safety profile in participants with psoriasis, but without reduction in skin infiltrate thickness or disease biomarkers.
Glycerides are the main components of oils, and fats, used in formulated products in the food and cosmetic industry as well as in the pharmaceutical product industry. However, there is limited literature available on the analysis of the chemical composition of glycerides. The lack of a suitable analytical method for complete chemical profiling of glycerides is one of the bottlenecks in understanding and controlling the change in chemical composition during processing, formulation, and storage. Thus, the aim of the present study is to develop a calibration-free quantitative proton nuclear magnetic resonance (qHNMR) method for the simultaneous quantification of different components of glycerides. The qHNMR method was developed for the quantification of mono-, di-, and triglycerides; their positional isomers; free fatty acids; and glycerol content. The accuracy, precision, and robustness of the developed method were evaluated and were found suitable for the quantitative analysis of five batches of marketed excipient. The study demonstrates the potential of qHNMR method for the quantification of different components of glycerides in various marketed products. The method has the ability to identify the variability of glycerides among different batches and suppliers in terms of chemical composition and also to discern the changes during storage.
The skin is an attractive site for immunization in humans and animals, owing to its resident population of dendritic cells and macrophages along with extensive vascularization by lymphatic vessels and blood capillaries. In addition to these physiological attributes, the intradermal route for vaccine delivery also presents a less-invasive alternative to conventional subcutaneous or intramuscular injections. This may offer compliance and convenience advantages for a wide range of stakeholders including patients, healthcare providers, veterinarians, animal owners and animal producers. This review discusses the current developments in intradermal vaccination for human and veterinary applications, with particular focus on the skin immunology, vaccine antigens and adjuvants and delivery systems.
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