The obtaining of bioactive di-and tripeptides using AlcalaseÒ and NeutraseÒ enzymes in the hydrolysis of soybean meal (SM) and its protein isolate (SPI) was evaluated. An innovative system by fast LC-MS/ MS neutral loss screening and de novo sequencing was used to identify bioactive peptides. Soy protein characterisation, gel electrophoresis and antioxidant activity of the obtained peptides were performed. Results achieved showed that the use of AlcalaseÒ and SPI preparation potentiated the peptide breaking bonds and favoured the obtainment of bioactive peptides. The antioxidant activity of tested samples was significantly improved with enzymatic hydrolysis. LC-MS/MS analyses identified nineteen peptides in SM and 51 in SPI, all obtained after hydrolysis with AlcalaseÒ and, according to BIOPEP, with relevant bioactivities and expressive functional potential. Therefore, it is suggested that bioactive peptides achieved in this study can enable the development of new ingredients and provide greater added value to soy byproducts.
The materials used were ethanol, soluble starch, commercial corn starch, tryptone, yeast extract, sodium carbonate, sodium chloride, and agar. β-cyclodextrin and HPLC grade acetonitrile were purchased from Sigma-Aldrich Ltda, São Paulo, Brazil. The antibiotics used were Kanamycin Sulfate and Hygromycin B., from Streptomyces hygroscopicus. All other reagents used were of analytical or chromatographic grade.
Methods
Cultivation and growth of the recombinant B. subtilis WB800 bacteriumFor the cultivation and growth of the bacteria, 2xYT medium (liquid and solid) was used, composed of 1.6% tryptone, 1% yeast
A crescente procura por alimentos funcionais e/ou saudáveis tem sido expressiva nos últimos anos. A banana, quando verde e cozida consiste, em grande parte, em amido resistente, este um alimento funcional do tipo prebiótico. O presente trabalho traz uma revisão bibliográfica integrativa do período dos últimos 5 anos nos seguintes bancos de dados: LILACS (Literatura Latino Americana e do Caribe em Ciências da Saúde), MedLine (Medical Literature Analysis and Retrieval System Online), SciElo (Scientific Eletronic Library Online). Com os seguintes descritores: biomassa, biomassa de banana verde, banana verde, alimento funcional. Foi aplicado um filtro para os artigos pertinentes ao tema. Posteriormente, foram descartados os artigos em duplicata, repetidos, publicados em mais de um banco de dados. Feita uma análise sistemática, as informações relevantes foram elencadas no presente trabalho.
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