SummaryThe decomposition of lipid hydroperoxides (LOOH) into peroxyl radicals is a potential source of singlet molecular oxygen (
The decomposition of peroxynitrite to nitrite and dioxygen at neutral pH follows complex kinetics, compared to its isomerization to nitrate at low pH. Decomposition may involve radicals or proceed by way of the classical peracid decomposition mechanism. Peroxynitrite (ONOOH/ONOO(-)) decomposition has been proposed to involve formation of peroxynitrate (O(2)NOOH/O(2)NOO(-)) at neutral pH (D. Gupta, B. Harish, R. Kissner and W. H. Koppenol, Dalton Trans., 2009, DOI: 10.1039/b905535e, see accompanying paper in this issue). Peroxynitrate is unstable and decomposes to nitrite and dioxygen. This study aimed to investigate whether O(2)NOO(-) formed upon ONOOH/ONOO(-) decomposition generates singlet molecular oxygen [O(2) ((1)Delta(g))]. As unequivocally revealed by the measurement of monomol light emission in the near infrared region at 1270 nm and by chemical trapping experiments, the decomposition of ONOO(-) or O(2)NOOH at neutral to alkaline pH generates O(2) ((1)Delta(g)) at a yield of ca. 1% and 2-10%, respectively. Characteristic light emission, corresponding to O(2) ((1)Delta(g)) monomolecular decay was observed for ONOO(-) and for O(2)NOOH prepared by reaction of H(2)O(2) with NO(2)BF(4) and of H(2)O(2) with NO(2)(-) in HClO(4). The generation of O(2) ((1)Delta(g)) from ONOO(-) increased in a concentration-dependent manner in the range of 0.1-2.5 mM and was dependent on pH, giving a sigmoid profile with an apparent pK(a) around pD 8.1 (pH 7.7). Taken together, our results clearly identify the generation of O(2) ((1)Delta(g)) from peroxynitrate [O(2)NOO(-) --> NO(2)(-) + O(2) ((1)Delta(g))] generated from peroxynitrite and also from the reactions of H(2)O(2) with either NO(2)BF(4) or NO(2)(-) in acidic media.
Prochilodus is one of the most important fish resources of South America, in addition to the important role it plays in nutrient cycling of Neotropical rivers. In the present study, we describe the isolation and characterization of nine novel microsatellite loci in Prochilodus argenteus. The number of alleles per polymorphic locus varied from 5 (Par76) to 21 (Par85), revealing a total of 116 alleles. The values of observed and expected heterozygosities ranged from 0.629 (Par69) to 0.926 (Par85 and Par86) and from 0.643 (Par66) to 0.931 (Par80), respectively. Furthermore, the ability of these and other previously described microsatellite markers to amplify orthologous loci was tested in two related species, Prochilodus costatus and Prochilodus lineatus. These loci will be useful for studies of population genetic structure in this group of fishes, and in aiding future genetic mapping studies of P. argenteus.Key words: cross-species amplification, enrichment libraries, microsatellite, Prochilodus. Family Prochilodontidae constitutes one of the most important fish resources of South America (Bayley and Petrere, 1989), in addition to its important role in nutrient cycling in Neotropical rivers (Flecker, 1996). Prochilodus comprises 49 nominal species of which only 13 species are valid (Castro and Vari, 2003). Among the species found in the São Francisco River basin, Prochilodus argenteus, popularly known as curimatã-pacu, forms a bulk of the subsistence fishery of the region, although numbers harvested have drastically declined in the last years (Sato and Godinho, 2004).Microsatellites are polymorphic DNA sequences containing short tandemly arranged repetitions (Tautz, 1989), distributed throughout the genome (Litt and Luty, 1989), and found in all prokaryotic and eukaryotic genomes studied until now (Zane et al., 2002). Due to their high variability, these genetic markers have been widely used in genetic mapping (Knapik et al., 1998;Shimoda et al., 1999;Coimbra et al., 2003) and population structure studies Primmer et al., 2006). However, one of the great impediments for the wider use of microsatellites is the need to isolate and characterize these markers via cloning and sequencing of genomic libraries for each species of interest (Angers and Bernatchez, 1997). Nevertheless, once the flanking sequences of microsatellite markers are known, a large number of individuals may be rapidly genotyped.Although the Neotropical ichthyofauna is the world's most diversified (Lowe-McConnel,1969;1987), microsatellite primers have been published only for Piaractus mesopotamicus (Calcagnotto et al., 2001), Astyanax fasciatus (Strecker, 2003), Arapaima gigas (Farias et al., 2003), Brycon opalinus (Barroso et al., 2003), Eigenmannia sp. (Moysés et al., 2005), Pseudoplatystoma corruscans (Revaldaves et al., 2005), Brycon hilarii (Sanches and Galetti, 2006) and Prochilodus costatus (Carvalho-Costa et al., 2006). The isolation and characterization of microsatellite loci has also been performed in P. argenteus, and thirteen loci ...
Prochilodus species inhabit the main river systems of South America and usually present commercial value to inland fishing. In the present study, we describe the isolation and characterization of 13 novel microsatellite loci in Prochilodus argenteus . The number of alleles per polymorphic locus varied from four to 22 and the observed heterozygosity ranged from 0.333 to 0.893. Additionally, cross-species amplification was successful in two other Prochilodus species. These loci will be useful for studies of the population genetic structure in this fish group.
ABSTRACT1. Waterbirds are increasingly affected by climate change and human disturbances to the wetlands on which they roost, forage and breed. The evolutionary response of populations to such changes is influenced by genetic variability and gene flow patterns, which enable long-term survival. Thus, genetic monitoring of waterbird populations can provide valuable information to support conservation measures and management policies for wetlands.2. This study assessed past and contemporary levels of genetic diversity, estimated effective population sizes (Ne) and investigated gene flow patterns among populations of the great egret, Ardea alba egretta, settled in major Brazilian wetlands.3. Samples (N = 200) were collected from the northern, central western, south-eastern and southern regions of Brazil. AMOVA, F-statistics, assignment tests, Bayesian clustering analyses and Ne were estimated based on mitochondrial DNA (mtDNA) and microsatellite loci.4. The populations share most mitochondrial haplotypes, suggesting a common recent past. Mismatch analyses, Fs and D statistics, and SSD and Rg indices indicated significant signs of expansion in most populations. The time since expansion suggests that egrets colonized southern latitudes more recently, probably accompanying the supposed historical environmental changes in South America, with more stable habitats toward equatorial regions.5. MtDNA Ф ST revealed significant differentiation between the northern and both the central western and southern populations. Nuclear loci demonstrated significant structuring between the central western and southern populations, which showed similar effective sizes.6. Despite the considerable dispersal potential of the great egret, there is limited gene flow among populations located in different Brazilian wetlands. Therefore, colonies from different regions should be preserved, with special attention to the northern populations, whose allelic constitution differs from the other. This approach can be used to genetically monitor similar species in other wetlands or to great egret populations in other regions of the Americas.
Certificamos que a proposta intitulada "Clonagem e expressão da proteína MCP recombinante de isolado brasileiro de Ranavirus e produção de anticorpos policlonais anti-MCP", protocolada sob o CEUA nº 2265041116 (ID 000491), sob a responsabilidade de Ricardo Luiz Moro de Sousa e equipe; Thaís Camilo Corrêa -que envolve a produção, manutenção e/ou utilização de animais pertencentes ao filo Chordata, subfilo Vertebrata (exceto o homem), para fins de pesquisa científica ou ensino -está de acordo com os preceitos da Lei 11.794 de 8 de outubro de 2008, com o Decreto 6.899 de 15 de julho de 2009, bem como com as normas editadas pelo Conselho Nacional de Controle da Experimentação Animal (CONCEA), e foi aprovada pela
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