Animal production systems and agribusinessFull-length research article Concerns, attitudes, and opinions of meat buyers in Garanhuns, Pernambuco, Brazil ABSTRACT -With the objective of identifying the concerns, attitudes, and opinions of meat buyers and their relationship with their choice of place of purchase (supermarkets, free fair, or butchers), 381 consumers in the city of Garanhuns, Brazil, were interviewed. Data analysis was performed using descriptive statistics and discriminant analysis, complemented by stepwise regression, Wilks' Lambda test, and Fisher's linear test.Most of the respondents expressed that inadequate commercialization of meat occurred in free fairs, and the lack of hygiene and the fact that meat was exposed in the environment without refrigeration were the main preoccupations. They also reported that meat consumption without inspection could lead to disease transmission, with pork being considered the most dangerous. Based on the theory of planned behavior, buyers agreed that their attitudes toward the purchase of meat (concern with food safety, price, animal welfare, environment, and slave labor) influenced their purchasing decisions. Regarding the subjective norms, the results indicated that purchase intention could be modulated by the opinion and judgment that other people exercise on the buyer's choice decision. Regarding perceived control, the respondents said that they were confused at the time of purchase and got irritated after making a purchase that did not satisfy their desires. The factors that differentiated consumers who prefer to buy meat in supermarkets from those who prefer butchers and free fairs are mainly the price of the product, custom/tradition, customer service, and hygiene of the establishment. Buyers who have a lower level of schooling and live in rural areas also tended to buy meat in free fairs.
In the present study, buffalo milk caseinate hydrolysates produced by bromelain, neutrase, papain and trypsin were ultra-filtered and different fractions were assessed for antioxidant, inhibition of angiotensin converting enzyme and antimicrobial activity. Biological potential was assessed by a number of metrics: ability to remove radicals of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyls; copper and iron chelation; antidiabetic properties; antihypertensive assay; and antibacterial activity against Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19114, Salmonella typhimurium ATCC 14028 and Staphylococcus aureus ATCC 25923 strains. The tests for scavenging of hydroxyl radicals and DPPH revealed a greater potential in the 3–10 kDa fractions. Iron chelation activity >70% was observed in all the fractions, including <3 kDa. Copper chelation was >60% in fractions >10 kDa. α-Amylase inhibition and antihypertensive activity was optimal in the <3 kDa fraction. Antibacterial activity ranged between 3.28 and 100% inhibition against microorganisms tested, the fraction <3 kDa showed a greater inhibitory potential. The antihypertensive activity of fractions ranged between 39.35 and 89.58%. All treatments were able to produce hydrolysates and fractions with biological potential and, so the ultrafiltration method proved to be effective in the separation of peptides with different molar masses and potential use in the food or pharmaceutical industry.
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