Adult neocortex contains dividing satellite glia population even though their characteristics and functions have still remained unknown. Nestin(+)/NG2(+) cells as major fraction of dividing glial cells express bicuculline-sensitive gamma-aminobutyric acid A (GABA(A)) receptors and receive GABAergic inputs. Due to their high [Cl(-)](i), GABAergic activation depolarized the cells and then induced Ca(2+) influx into them. To assess an effect of this GABAergic excitation, we looked for the expression of neurotrophic factors. Among them, we detected the expression of brain-derived neurotrophic factor (BDNF) on the cells. The level of BDNF expression was elevated after cortical ischemia, and this elevation was blocked by bumetanide, an inhibitor for NKCC1 that blocks the GABAergic depolarization. Furthermore, performing a modified adhesive removal test, we observed that the treatment of bumetanide significantly attenuated the recovery in somatosensory dysfunction. Our results may shed a light on satellite glia population in the cortex and imply their roles in the functional recovery after ischemic injuries.
A C 36 linear acetylene alcohol, lembehyne A (LB-A), induces neuronal di¡erentiation against neuroblastoma cells morphologically and also functionally. The di¡erentiation and cytostatic e¡ect induced by LB-A was speci¢c to neuroblastoma, Neuro 2A cells. To identify the target protein for LB-A, a radioactive photoa⁄nity probe, [125 I]18-(2P P-azido-5P P-iodo-benzoyloxy)-LB-18 ([ 125 I]azido-LB-18), was synthesized. As a result of in situ labeling experiments against Neuro 2A cells, a protein of M r 30 kDa was photolabeled speci¢cally. This labeling was inhibited in the presence of LB-A or the active analogs of LB-A, whereas the inactive analogs showed no inhibitory e¡ect on this labeling. These results suggest that this protein of M r 30 kDa is the target protein for LB-A and may play an important role for the neuronal di¡erentiation in neuroblastoma, Neuro 2A cells. ß
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