Temperature is one of the main abiotic factors affecting the development of causative agents of nosemosis in the bee's body. The aim of the research. To determine the influence of the winter and summer bee nest temperature (20–22 °C and 35–36 °C, respectively) on the duration of Nosema species development of winter bee generation isolated in hoarding cages, and to trace the life cycle of Nosema species of bees with natural infection and overwintering in natural conditions, from November to the beginning of bee brood rearing (February). Materials and methods. For this, 200 bees Apis mellifera sossimai, selected from bee colony in November, were infected by syrup feeding containing Nosema species (5×104 spores per bee). Half of the bees were kept at 35–36 °C and half at 20–22 °C. The stages of Nosema species development were monitored daily for 13 days in midgut smears, stained according to Romanovsky-Giemsa (magnification 900x). Furthermore, with a 15 days frequency, from November to mid-February, 30 bees were selected from 20 bee colonies overwintered in natural conditions, and monitored the stages of Nosema species development at natural infection. Result. It was found that the Nosema species development of winter bee generation artificially infected by Nosema species, was suspended at meronts and sporonts stages until the 13th day from the moment of infection, regardless of the temperature at which the bees were kept in the experiment. In bees selected from bee colonies naturally infected with Nosema species prevailed meronts I, II and in an insignificant amount sporonts, until the end of December, active sporulation took place from the middle of January to the beginning of February. Conclusion. That is, the duration of the life cycle development of Nosema species depends little on temperature but is closely related to the life span of summer and winter bee generation and determined by the biochemistry of their relationships, which allow the parasite to save the host as its habitat
Ukraine's economy needs in oil and gas consumption are not currently decreasing, and in connection with military actions in the east of the country, they are rather growing, so there is an urgent need for our country to increase energy resources. In this regard, it is necessary to accelerate the processes of hydrocarbon deposits exploration. Today, as much as before, the most promising area for exploration of new oil and gas deposits remains the Dnipro-Prypyat oil and gas-bearing province, in particular the Dnipro-Donetsk Basin. The article is devoted to urgent issues regarding forecasting onshore oil and gas prospects based on aerospace and geology methods.In the process of approbation of the proposed technology with materials of space and geological research, the first-priority areas, which are recommended for conducting detailed seismic exploration works, have been selected on the Lyvenska territory. The results of drilling operations within these areas will make it possible to assert the high reliability of the provided forecasts.
This detailed investigation was conducted for the South part of the Orikhovo-Pavlograd suture zone by using satellite imagery. At the first stage, the imagery was used to extract lineaments and arcuate relief elements. Further, geomorphological anomalies were highlighted by means of structural and geomorphological analysis of the study area. The significant number of ultrabasite bodies were distinguished alongside the entire Orikhovo-Pavlograd suture zone with allowance for the geological structure. The sites of kimberlite magmatism defined according to geophysical investigations can be associated with ultrabasite bodies. Prognostic sites of kimberlite magmatism were allocated and complex of morphostructural geoindication features were educed above the prognostic paleo-volcano that had erupted ultrabasites. The geoindicators of morphostructures are: the spreading and narrowing of a floodplain; displacement of a stream bed; deeping of the stream bed plunging depth; straightened reach of the stream bed; discordant course of the left tributaries of the Molochna river; augmentation of the stream bed meandering; floodplain marshy parts at the beginning of morphostructures; intense developing of a ravine-gully network on the right side of the Molochna river; localization of sand massifs on the anomalously located second terrace of the Molochna river flood-plain; rills and numerous artesian wells within the floodplain. These indicators have made it possible to construct a geoindication scheme, distinguish the prognostic Melitopol morphostructure and make a prognosis for mineral exploration. The formation of this morphological structure can be ascribed with a paleo-volcano, which igneous rocks are ultrabasites and kimberlites. Rocks of these complexes can be prospective evidence for diamond, iron, nickel, copper, lead and zinc deposits exploration. Hydrothermal and metasomatic sulphide (polymetallic) mineral occurrences can be explored as well. The procedure of geoindication investigation will be applied for integrated exploration throughout the entire area of the Orekhovo-Pavlograd suture zone.
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