Dysbiotic disorders of the gastrointestinal tract (GIT) are one of the urgent problems today. There are a number of directions and means aimed at restoring the normobiota of the GIT, comprising of probiotics. An alternative to them can be probiotics and nutraceuticals, which are used in cases when the application of live strains is contraindicated. The purpose was to assess the possibility of using Nigella sativa oil to eliminate antibiotic-associated dysbiosis disorders. Methods: Dysbiosis was modeled by administering of ampicillin (75 mg/kg) and metronidazole (50 mg/kg) to non-linear male rats (170-200g, n=49) for 7 days. N. sativa «Diana» variety oil (200 mg/kg) was administered to the animals after antibiotics withdrawal. In comparison were used oil: N. sativa «Ancient Traditions», Ukraine (200 mg/kg); N. sativa «Messenger's Speech», Egypt (200 mg/kg), home-pressed sunflower oil (200 mg/kg); prebiotic «Healthy Tract Prebiotic» (30 mg/kg). All preparations were administered per os for 7 days. The quantitative composition of the microbiota was determined by plating 10-fold dilutions of fecal biopsies on elective media, expressed in lg (m+M) CFU/g. Results: The study has demonstrated a significant decrease in Lactobacillus and lactose-positive E. coli by 2 orders as well after antibiotic modeled dysbiosis. On the contrary, there was a significant increase in the number of lactose-negative E. coli by 4 orders and conditionally pathogenic enterobacteria (CPE) by 3 orders. Administration of N. sativa «Ancient Traditions» and «Messenger's Speech» to animals contributed to the complete restoration of the disturbed microbiota. The use of prebiotic partially contributed to the restoration of lactobacilli and lactose-positive E. coli titers, but didn`t eliminate the changes in lactose-negative E. coli and CPE. N. sativa«Ancient traditions» and sunflower oil did not have a positive effect on the restoration of the GIT microbiota at all. Conclusion: The use of N. sativa «Diana» variety oil for 7 days contributes to the complete restoration of the normobiota disturbed by the administration of antibiotics.
Background: Currently, diseases of the urinary system are observed in 3.5–4% of the world's population. According to WHO, the number of people suffering from this pathology doubles every 7–10 years. To date, hyperoxaluria is considered as the main risk factor for the formation of oxalate-calcium stones, which account for 75% of all kidney stones. One of the main causes of hyperoxaluria is a decrease in the number of microorganisms capable of degrading oxalates, which occurs due to the disruption of the intestinal microbiota. Oxalate-degrading bacteria include the genera Lactobacillus, Bifidobacterium, Oxalobacter formigenes etc. Searching of probiotic strains with high oxalate-degrading activity have become one of the priorities from the context of research. The aim of the present study was to isolate Lactobacillus spp from different sources and to determine their ability to degrade oxalate. Methods: A total of 23 Lactobacillus spp. from food of animal and vegetable origin were isolated with selective MRS Broth medium and further cultured on MRS Agar or Oxalate Medium with 5 g/l sodium oxalate. ANAERO test23 was used to assess the species affiliation. Oxalate-degrading activity (ODA) was measured by redox titration with KMnO4. Results: Only 7 species of isolated bacteria out of 23 showed the ability to grow on the oxalate-containing culture medium. According to the morphological and physiological-biochemical characteristics, these bacteria belonged to the genus Lactobacillus: L. nagelii – 2 spp, L. rhamnosus – 2 spp., L. frumenti - 1 spp, L. plantarum – 1 spp, L. acidophilu – 1 spp. The most active metabolizers of oxalate on Oxalate Medium were L. plantarum S3 – 42%; L. acidophilus S5 – 38%, and L. nagelii Z2 – 35%; the worst results were shown by L. rhamnosus K7 and L. nagelii S12 – both metabolized only 7% of sodium oxalate. Conclusions: The redoximetric titration with KMnO4 was adopted to evaluate the ODA of bacteria in culture media. Lactobacillus spp. isolated from different sources differs according to the level of ODA. Three promising Lactobacillus species were selected for further estimation of probiotic profile.
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