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THE DIAGNOSIS of gonorrhea in the fe¬ male is notoriously difficult and fallible. New and reliable diagnostic methods are urgently needed if we are to control the disease.A new commercial culture medium for the primary isolation of Neisseria gonorrhoeae has been developed by the Baltimore Biological Laboratory (BBL). This medium differs from other conventional commercial chocolate media in that the added enrichment, instead of being blood or yeast extract, is chemically defined (1).A trial of this medium was made using speci¬ mens from three groups of patients: females who were conjugal contacts to males with proven gonorrheal urethritis, females who entered the clinic as self-referrals, *and males with acute gonorrheal urethritis. MethodsThe efficacy of the medium was judged on cultures of gonococci from urethral and vaginal specimens. Two base media were used: Difco chocolate medium, Difco Laboratories, Detroit, Mich., and BBL chocolate medium, prepared according to the directions of the manufac¬ turers. Bacto supplement B, a yeast extract, was used to enrich the Difco basal medium. The BBL chocolate medium was enriched with the chemically defined supplement "IsoVi-taleX" (1), Which contained vitamin B-12, 1-glutajnine, adenine, guanine, p-aminobenzoic acid, 1-cystine, glucose, diphosphopyridine

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