We present hereafter a protocol to test attractiveness of new formulations of granulated baits used against leaf-cutting ants (Acromyrmex spp. and Atta spp.) in the field. Its purpose is to standardize the methodology, thus making possible further comparison of results wherever these tests are carried out.
Th e exocrine metapleural gland is unique for ants and is characterized by antibiotic production. Th is work aimed at comparing (1) the volume of the metapleural gland, (2) the nucleus-cytoplasm relation of the secretory cells and (3) the surface area of the bulla which corresponds to the external part of the gland in workers of Atta sexdens rubropilosa Forel, 1908 . Th e measurements were conducted in medium-sized workers of the following groups: waste processors (workers that manipulated waste), waste removers (workers that transported the waste fragment), foragers and workers inside the fungus garden. We show that the volume of the metapleural gland reservoir is larger in groups that are more exposed to infections.
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