Multifunctional interleukin 10 (IL10) + Th1 cells have been implicated in favorable evolution of many infectious diseases, promoting an efficacious immune response while limiting immunopathology. Here, we investigated the presence of multifunctional CD4 + and CD8 + T-cells that expressed interferon gamma (IFNc), IL10 and tumor necrosis factor (TNF), or its combinations during dengue infection. Peripheral blood mononuclear cells (PBMCs) from outpatients with dengue (mild dengue forms) and hospitalized patients (or patients with dengue with warning signs and severe dengue) were cultured in the presence of envelope (ENV) or NS3 peptide libraries of DENV during critical (hospitalization period) and convalescence phases. The production of IFNc, IL10 and TNF by CD4 + and CD8 + T-cells was assessed by flow cytometry. Our data show that patients with mild dengue, when compared with patients with dengue with warning signs and severe dengue, presented higher frequencies of multifunctional T-cells like NS3-specific IFNc/IL10-producing CD4 + Tcells in critical phase and NS3-and ENV-specific CD8 + T-cells producing IFNc/IL10. In addition, NS3-specific CD8 + T-cells producing high levels of IFNc/TNF and IFNc/TNF/IL10 were also observed in the mild dengue group. We observed that multifunctional T-cells produced higher levels of cytokines as measured by intracellular content when compared with single producer T-cells. Importantly, multifunctional CD4 + and CD8 + T-cells producing IFNc, TNF and IL10 simultaneously displayed positive correlation with platelet levels, suggesting a protective role of this population. The presence of IL10 + Th1 and IL10 + Tc1 multifunctional cells was associated with mild dengue presentation, suggesting that these cells play a role in clinical evolution of dengue infection.
Preeclampsia is an obstetric disease of unknown cause that affects approximately 5% of pregnant women. The visual system may be affected with variable intensity, being the retinal detachment a rare complication. The retinal detachment in preeclampsia is usually bilateral and serous, and its pathogenesis is related to the choroidal ischemia secondary to an intense arteriolar vasospasm. The majority of patients have complete recovery of vision with clinical management, and surgery is unnecessary. This is a case report of a 27 year old patient who developed the severe form of preeclampsia on her first pregnancy. She had progressive blurred vision, until she could see only shadows. Ophthalmic examination diagnosed spread and bilateral retinal detachment. With blood pressure control at postpartum, the patient had her retina reattached, and recovery of vision.
O envelhecimento populacional é uma realidade que chega imbuída de complicações as quais muitas vezes tornam-se problema de saúde pública, como é o caso das quedas hospitalares. Estas acarretam uma série de conseqüências negativas ao paciente e sua família, bem como ao sistema de saúde. O objetivo deste estudo foi avaliar e quantificar, por meio de entrevistas com os profissionais responsáveis pelos cuidados dos pacientes da Santa Casa de Belo Horizonte, a ocorrência de quedas durante a hospitalização. Foram entrevistados 37 profissionais (médicos, residentes, enfermeiros e prestadores de serviços) através de questionário semi-estruturado com 11 questões referentes ao histórico de quedas no setor, englobando informações sobre número de episódios, local, horário, idade do paciente, atividade que realizava no momento, conseqüências, bem como questões referentes à adaptação ambiental. A análise dos dados feita por meio de estatísticas descritivas. Os resultados mostraram que 65% dos funcionários relataram a ocorrência de quedas. A maioria dos caidores (73%) eram homens, com média de idade de 78,62 anos (DP +- 7,63) e caíram mais nos períodos da manhã (31%) e noite (31%), principalmente no quarto (48%) e no banheiro (42%) sendo que 50% dos episódios não foram injuriosos. Conclui-se que os episódios de quedas ocorrem no ambiente hospitalar, mas não são reportados de maneira adequada, tornando necessário adoção de medidas educativas aos profissionais que lidam com esta população.
Estudo clínico comparativo e ultra-sonografia entre indivíduos negativos e positivos para Schistosoma mansoni em áreas endêmicas de baixa morbidade e pacientes hospitalizados com doença hepatosplênica G2 and G3, respectively, in 11.1, 12.1 and 26.3%. In G1, G2 and G3, periportal with periportal echogenicity (3-6mm); and G4 -9 hepatosplenic patients with periportal echogenicity (> 6mm). Hepatomegaly detected by physical examination of the abdomen evaluated in the midclavicular line was verified in G1,
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