An experimental calcium silicate-based root-end filling material incorporated with silver nanoparticles intended for use in periapical surgeries was developed with the purpose to overcome the drawbacks of existing materials and to satisfy the ideal requirements of root-end filling materials. This study was designed to evaluate the physicochemical properties, pH, calcium ion release, and dimensional stability of the experimental cement, and compare the results with commercially available ProRoot MTA (Dentsply). An independent sample test was used to analyze the data. Mean initial pH (immediately after mixing) of the experimental cement was 10.42 ± 0.04 which was higher than that of MTA. However, there was a significant increase in pH of MTA at 1 day, 2 days, and 7 days. Presence of calcium chloride favored the release of calcium ions which was significantly increased in the experimental group at 24 hours. At the end of 30 days, MTA showed a significant expansion when compared to the experimental cement p < 0.001 . In conclusion, the experimental nanoparticle-incorporated calcium silicate-based cement showed clinically acceptable physicochemical properties.
Aim:The aim of the study was to evaluate the cytotoxicity of mineral trioxide aggregate (MTA) when mixed with either silver or titanium dioxide (TiO 2 ) nanoparticles on human lymphocytes. Materials and methods: Human lymphocytes were exposed to white MTA or MTA in combination with silver or TiO 2 nanoparticles. The cytotoxicity was assessed using the MTT assay for freshly mixed pellets, at 24 hours, at 48 hours, and at 72 hours time periods. The results were statistically analyzed. Results: There was no significant difference in the experimental groups with regard to cell viability. The addition of nanoparticles to MTA showed similar cell viability compared with MTA alone. Conclusion: Mineral trioxide aggregate mixed with silver or TiO 2 nanoparticles showed similar biocompatibility to MTA alone. Clinical significance: The cytotoxicity exhibited by silver or TiO 2 nanoparticles when mixed with MTA showed no significant difference and therefore, they can be used as a suitable additive to improve the antimicrobial efficacy of MTA.
Background Although several materials have been used for retrograde filling following apical surgeries, there is no consensus on a single best material. Tricalcium silicate-based types of cement have been developed as root-end filling materials mainly due to tricalcium silicate’s hydraulic properties. However, its unfavorable setting characteristics and minimal antimicrobial properties have necessitated the introduction of new additives into the existing commercially available materials. To design an affordable product based on a dicalcium silicate with a shorter set time, minimal cytotoxic complications, and enhanced antibacterial activity, we developed a new endodontic cement from pure raw materials, intending to satisfy the prerequisites of ideal retrograde material. Methods The composition of the experimental calcium silicate-based cement included the addition of calcium chloride and silver nanoparticles in varying concentrations. Structural characterization was carried out using energy dispersive analysis by X-rays using scanning electron microscope (EDAX SEM) and hydration characteristics were performed using an X-ray diffractometer (XRD). The experimental material was further evaluated for biocompatibility using MTT ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide)assay and antibacterial activity was evaluated using an agar diffusion test against Enterococcus faecalis. Results The structural characterization and hydration characteristics revealed that the experimental cement was dicalcium silicate based with favorable biocompatibility and enhanced antibacterial activity. Tricalcium silicate based mineral trioxide aggregate (MTA) also had favourable biocompatibility, however, its antibacterial activity was significantly decreased when compared to the novel cement. Conclusion All hydraulic cements that are available in the dental market are predominantly tricalcium silicate-based materials. There has been no evidence in the literature to date wherein it has been explored whether a dicalcium silicate-based hydraulic cement can solely be used in root-end cavities. The findings of the study revealed a dicalcium silicate based retrograde filling material with favourable biocompatibility exhibited immediately as well as in the set samples. Incorporation of silver nanoparticles boosted the antibacterial activity when compared to that of ProRoot MTA. This material could potentially reinstate the usual hype created with tricalcium silicate types of cement since dicalcium silicate cements also exhibit similar properties.
Background Mineral trioxide aggregate (MTA) exhibits several properties of an ideal root-end filling. It, however, has certain disadvantages such as long setting time, difficult handling characteristics, and limited antimicrobial activity. In order to improve its properties, several commonly used reagents have been used instead of sterile water. Hence the objective of this study was to evaluate the root-end sealing ability of white MTA combined with either distilled water, 0.12% chlorhexidine solution, 10% doxycycline solution, 3% sodium hypochlorite solution, or 10% calcium chloride solution using a bacterial leakage test. Materials and methods Seventy-two extracted, decoronated human maxillary incisor teeth were used. After cleaning and shaping of the canals, root-end resection was performed at 90°. Root-end cavities were filled with ProRoot MTA combined with the test reagents. Microleakage was assessed using a bacterial leakage test for a period of 60 days. Results were analyzed using the log rank test. The level of significance was 0.05. Results There was a significant increase in the sealing ability when MTA was mixed with calcium chloride, sodium hypochlorite, or doxycycline when compared with distilled water or chlorhexidine. Also, MTA combined with calcium chloride demonstrated a higher probability of surviving 60 days. Conclusion The sealing ability of MTA was improved when combined with calcium chloride, sodium hypochlorite, and doxycycline. How to cite this article Dsouza T, Bhandary S, Srinivasan R. An in vitro Comparative Evaluation of the Sealing Ability of Mineral Trioxide Aggregate with Different Mixing Reagents. World J Dent 2016;7(2):54-58.
The evolution of calcium silicate based cements in the field of dentistry has been rewarding. It offers a plethora of advantages with respect to physicochemical and biological properties. However, the extended setting time and limited antibacterial activity in these cements is quite a concern. This study aimed to develop a fast-setting nanoparticle incorporated root-end filling material for use after periapical surgeries. With the incorporation of the silver nanoparticles, and using calcium chloride as the liquid component, the desirable study outcome was achieved. The setting time was significantly reduced when compared to the commercially available root-end filling material.
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