. Symptomatic genitourinary medicine (GUM) . Asymptomatic GUM . Symptomatic primary care . Asymptomatic primary careMethods The Aptima TV NAAT test was performed on 9241 samples from women undergoing chlamydia and gonorrhoea NAAT testing in GUM and primary care. Results The positivity of TV determined by TV NAAT was 4.8% (26/543) and 1.8% (28/1593) in women with and without symptoms attending GUM and 2.7% (95/3512) and 1.1% (41/3593) respectively in primary care. TV positivity rates were high, as expected, in those of black ethnicity attending GUM (15.5% in those with symptoms). However TV positivity rates in primary care varied by practice (0-5.8%) in a way that could not be attributed to ethnicity alone. Conclusion This is the first study to report TV positivity, using a TV NAAT, in unselected women presenting for STI testing in primary care. Positivity proportions were higher than anticipated based on conventional testing methods particularly for symptomatic women in primary care. In view of the wide variation in TV positivity by locality, other factors e.g. deprivation may be important. This should be taken into consideration should targeted testing for TV be found to be cost effective, as targeting by ethnicity alone may miss cases. Disclosure of interest statement Hologic provided the tests for the Aptima TV NAAT research study and have sponsored the authors to present this data at ISSTDR. Background Rescreening women for Trichomonas vaginalis (TV) post treatment is important as repeat infections are common, ranging from 5%-31%. Nucleic acid amplification testing (NAAT) too soon after treatment may result in false positive results due to detection of remnant TV nucleic acids. The goal of this study was to determine the rate of false positive NAAT results at weeks 1-4 post treatment completion using culture as the gold standard. Methods Women attending an STI clinic in New Orleans who were InPouch culture positive and treated with metronidazole (MTZ) were included. Participants were scheduled for 4 weekly follow up visits beginning one week post-treatment completion. They provided self-obtained vaginal swabs (SOVS) and information regarding sexual exposure at each visit. SOVS were tested using InPouch culture and the Gen-Probe AptimaTV (GPATV) assay which targets ribosomal RNA. Women who were culture positive at follow-up were considered re-infected/treatment failure and were not followed further. Results 39 women were InPouch+ at baseline and were followed. Of these, 3 (7.7%) were InPouch TV+ at follow-up (1 at 1 week and 2 at 2 weeks) and reported no sexual exposure. Thus, these women were considered to be treatment failures and were no longer followed. Of the remaining cases, 5/29 (17.2%) were GPATV+ at the 1 week follow up visit, and 1/34 (2.9%) was GPATV+ at 2 weeks. The six positive women denied vaginal sexual re-exposure. None of the women were InPouch TV culture positive at any of the follow up visits and no woman was GPATV+ at 3 and 4 weeks post treatment.
O10.2 TRICHOMONAS VAGINALIS NUCLEIC ACID C...
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