A primary 3-dose hepatitis B (HB) vaccination series induces protective serum levels of antibodies to HB surface antigen (antiHBs >10mUI/mL) in 90% of healthy adults and 95% of children and adolescents. A 2 nd full-course vaccination induces protection in >50% of those who did not respond to the 1 st vaccination series Healthcare workers frequently do not perform post-vaccination anti-HBs testing. Although HB vaccination has been recommended for HCW since the 1980s, routine post-vaccination testing has not yet been incorporated in the care of HCW in Brazil. Additionally, the adherence of HCW to vaccination and post-vaccination testing is poor. Furthermore, the frequency of HCW who were vaccinated when they were children or adolescents is increasing, and post-vaccination testing is not recommended in routine HB immunization.In the management of HCW who are anti-HBs-negative after a full-course vaccination, the first step is to check the interval between
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