<p> <b><span style="font-size:10.0pt;">The <i>Tityus serrulatus </i>scorpion is considered the most dangerous scorpion in Brazil and is responsible for several cases of human envenomation annually. In this study, we performed transcriptome profiling of the <i>T. serrulatus</i> venom gland. In addition to transcripts with housekeeping functions, such as those related to protein synthesis, energy supply and structural processes, transcripts from thirty-five families of venom peptides or proteins were identified. These transcripts included three new complete sequences of toxins and more than a dozen putative venom gland proteins/peptides. The venom gland transcriptome profile was verified by comparison with the previously determined proteomic profile. In conclusion, this transcriptome data provides novel insights into the putative mechanisms underlying the venomous character of <i>T. serrulatus</i>. The collected data of scorpion transcripts and proteins/peptides described herein may be an important resource for identifying candidate targets of molecular therapies and preventative measures.</span></b><b><span style="font-size:10.0pt;"></span></b> </p>

not clear whether these multiple alleles represent separate translocation events or aneusomy of a previously translocated chromosome. The latter possibility is favored, given that: (1) the partner status (7q32.3 or other) was identical in the allele pairs from each case; (2) two of the three cases demonstrated aneuploidy; and (3) 6p25.3 translocations appear to be an early event during lymphomagenesis, based on our constant finding of this translocation in initial biopsies from patients with multiple tumor specimens over time (AL Feldman, unpublished observation). In addition to the lack of a normal copy of the 6p25.3 allele, multiple copies of the translocations could have implications on the partner locus. For example, we previously reported overexpression of microRNAs (especially MIR29B1) that reside near the 7q32.3 breakpoint in ALKnegative ALCLs with t(6;7)(p25.3;q32.3) (Feldman et al., 2009). In summary, we present three cases of pcALCL with biallelic rearrangements of 6p25.3 and no intact copies of the DUSP22-IRF4 locus. These cases indicate one mechanism by which both copies of the DUSP22 gene can be disrupted or deleted in T-cell lymphomas, and provide further evidence that DUSP22 may represent a tumorsuppressor gene.

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ABSTRACT. We used complex hypervariable repeats to evaluate the genetic diversity and structure of Prochilodus costatus (Characiformes), an ecologically and economically important species endemic to the São Francisco River basin. Hydroelectric dams along the river have led to population fragmentation, which can limit gene flow. Restocking from hatcheries has been used to repopulate declining populations. To determine how fragmentation and hatchery supplementation affect P. costatus population structure, we studied populations from three sites up and downstream of the Gafanhoto Dam (Pará River, State of Minas Gerais). High levels of genetic diversity were found within populations (0.926 to 0.873); the three populations showed significant differentiation (F ST = 0.16), suggest- 4457©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 4456-4467 (2012) Genetic diversity and structure in Prochilodus costatus ing that populations from the three sites were affected by fragmentation of the river and by hatchery contributions. These results will be useful for developing a management and conservation plan for fish species in this area.

Curimatã-pioa (Prochilodus costatus) and curimatã-pacu (Prochilodus argenteus) are migratory fish species endemic to the São Francisco River Basin in Brazil. Both species play important roles in local fisheries and ecology in the Paraopeba River. A dam was recently constructed on this river and to help in the development and conservation programs, we characterized the genetic variation of both species before dam construction. Complex hypervariable repeats microsatellite was used to asses genetic variation for both species within and between the five collection sites in order to detect population substructuring. Nucleotide substitutions and insertion/deletion polymorphisms (indels) resulted in 35 P. costatus haplotypes (sample size = 89) and 22 P. argenteus haplotypes (sample size = 32). Significant genetic diversity and population differentiation was detected between five sampling sites for both species. Therefore, each of the five sites should be regarded as a group comprising significant genetic differences in species conservation and maintenance plans. Comparing these results to genetic diversity measures after dam construction will be critical for future management in this region.

In recent decades, Brazilian hydroelectric reservoirs have been stocked with hundreds of millions of juvenile migratory fish, but almost no research on stocking has been conducted. In order to study stocking it is essential to tag the fish to be stocked. In this investigation we determined handling-induced mortality, tag retention rate, and absolute growth rate of hatcheryreared juvenile curimbatá (Prochilodus lineatus) tagged with coded wire tags (CWT), a tag widely used on stocked fish. We injected CWT into the dorsal musculature just below the first dorsal fin ray. Despite the need for frequent tag injector maintenance, this tagging location proved to be suitable for tagging juvenile curimbatá. We found no short-term mortality of juvenile curimbatá handled for CWT tagging, high retention rate for CWT (≥ 98.2%) even after 174 d of tagging, and no influence on absolute growth rate by CWT. We conclude that CWT is effective at tagging juvenile curimbatá, and we expect that it will also be effective for many other Brazilian fish species used for stocking.Nas últimas décadas, reservatórios de hidrelétricas do Brasil foram estocados com centenas de milhões de jovens peixes migradores, mas quase nenhuma pesquisa sobre a estocagem foi conduzida. Para estudo da estocagem, etiquetar os peixes a serem estocados é essencial. Neste estudo, nós determinamos a mortalidade devido ao manejo, taxa de retenção da marca e taxa de crescimento absoluto de juvenis de curimbatá de cativeiro (Prochilodus lineatus) marcados com 'coded wire tag' (CWT), etiqueta amplamente utilizada em peixes estocados. Nós injetamos o CWT na musculatura dorsal logo abaixo do primeiro raio da nadadeira dorsal. Apesar de exigir manutenção frequente do injetor de CWT, esse local mostrou ser apropriado para a marcação de jovens de curimbatá. Não houve mortalidade de curto prazo nos juvenis manejados para marcação com CWT, a taxa de retenção do CWT foi elevada (≥ 98,2%), mesmo após 174 dias da marcação. Além disso, o CWT não influenciou na taxa de crescimento absoluto dos peixes etiquetados. O CWT mostrou ser efetivo para marcar juvenis de curimbatá, e acreditamos que ele também o será para muitas outras espécies de peixes brasileiros utilizadas em estocagem.