Shiitake mushroom [Lentinula edodes (Berk.)Pegler] is a palatable fungus which derived its name from two words: "Shii" which means oak tree and "take" means mushroom. This mushroom contains important polysaccharide "lentinan" which exhibits many medicinal properties. The synthetic log method for the cultivation of shiitake mushroom was developed in the Fujian Province of China in 1986. With the development of this method, China was able to dominate the market and is currently known as the largest producer of Lentinula edodes (Singh et. al., 2020). The present investigations were planned to produce synthetic logs of different substrates and their combinations to evaluate the effect of shock treatment with ice cold water on them.
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