Surface-exposed loop residues, Pro(389) (beta(6)-beta(7) loop), Glu(417) (beta(8)-beta(9) loop), Tyr(455) and Asn(456) (beta(10)-beta(11) loop), in the receptor-binding domain of the Bacillus thuringiensis Cry4Ba toxin have been previously demonstrated to be crucial for toxicity. Herein, five combinations of two-loop mutants, P389A/E417A (beta(6)-beta(7)/beta(8)-beta(9) loops), P389A/Y455A, P389A/N456A (beta(6)-beta(7)/beta(10)-beta(11) loops), E417A/Y455A and E417A/N456A (beta(8)-beta(9)/beta(10)-beta(11) loops), were constructed as a means of examining a correlative effect of these three critical loops on Cry4Ba toxicity. All 130-kDa mutant protoxins were overexpressed as inclusion bodies in Escherichia coli with yields comparable to the wild-type toxin. In addition, all mutant toxins were structurally stable upon solubilization and trypsin activation in carbonate buffer, pH 9.0. Interestingly, E. coli cells expressing all the double-loop mutants showed an almost complete loss in toxicity against Aedes aegypti mosquito larvae while their corresponding single-loop mutants exhibited reduced activity of approximately 50%. Moreover, in situ binding analysis revealed that the 65-kDa purified toxins representing each pairwise mutant exhibited reduced binding to apical microvilli of A. aegypti larval midgut when compared with the single mutants. Altogether, the data demonstrate for the first time that all these three surface-exposed loops of the Cry4Ba toxin are equally involved in receptor binding and hence toxicity.
Sodium chloride, commonly known as table salt, is widely used as essential seasoning in food, snacks and deserts worldwide. However, excessive consumption of table salt is a major cause of various health issues, involving high blood pressure, liver cirrhosis, kidney disease, and heart failure. This research aims at creating a portable, low-powered, efficient sensor for detection of sodium ions in body plasma for medical diagnosis purpose. The device was fabricated on a platform of Ion-Sensitive Field-Effect Transistor (ISFET) modified with sodium ionophore (sodium recognizing element), entrapped in polyurethane thin film. Our preliminary studies show that sodium ionophore-modified ISFET sensor yields good sensing performances, having a maximum sensitivity of 43 mV/pNa, and a detection limit of 2.3 millimol/liter.
OBJECTIVE: Mental stress is a major risk factor of metabolic diseases and impairs quality of life in the elderly. The goal of this study was to investigate whether a mindfulness-based intervention can improve health parameters and reduce stress-related hormones in elderly Thais. METHODS: A total of 192 participants were recruited from urban (n = 96) and rural areas (n = 96) in central region of Thailand. Creative visual art relaxation and meditative movement exemplified by Dao De Xin Xi exercise were introduced as monthly workshops and individual participants were encouraged to practice regularly for three months. General health status, levels of blood pressure (BP), fasting blood sugar (FBS), cortisol and dehydroepiandrosteone (DHEA) were evaluated before and after completing the intervention. RESULTS: This mindfulness-based intervention was effective as assessed by several biological health parameters. However, the degree of effectiveness differed between participants in urban and rural groups. In urban group, BPs were significantly reduced in hypertension and pre-hypertension subgroups of participants, and levels of DHEA in low DHEA participants were significantly increased after the intervention. In contrast, FBS levels in diabetic and pre-diabetic participants in the rural group, but not in the urban group, were significantly decreased after the intervention. We found a trend toward improvement of cortisol levels in both groups. However, the levels of glycated hemoglobin in either group remained unchanged. CONCLUSION: This mindfulness-based intervention to elderly people improved health parameters related to metabolic diseases. Therefore, applying the intervention in primary healthcare may help promote the well-being in elderly.
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