Telomeres regulate DNA damage response (DDR) and DNA repair activity at chromosome ends. How telomere macromolecular structure contributes to ATM regulation and its potential dissociation from control over non-homologous end joining (NHEJ)-dependent telomere fusion is of central importance to telomere-dependent cell aging and tumor suppression. Using super-resolution microscopy, we identify that ATM activation at mammalian telomeres with reduced TRF2 or at human telomeres during mitotic arrest occurs specifically with a structural change from telomere loops (t-loops) to linearized telomeres. Additionally, we find the TRFH domain of TRF2 regulates t-loop formation while suppressing ATM activity. Notably, we demonstrate that ATM activation and telomere linearity occur separately from telomere fusion via NHEJ and that linear DDR-positive telomeres can remain resistant to fusion, even during an extended G1 arrest, when NHEJ is most active. Collectively, these results suggest t-loops act as conformational switches that specifically regulate ATM activation independent of telomere mechanisms to inhibit NHEJ.
To investigate how chromatin architecture is spatiotemporally organized at a double-strand break (DSB) repair locus, we established a biophysical method to quantify chromatin compaction at the nucleosome level during the DNA damage response (DDR). The method is based on phasor image-correlation spectroscopy of histone fluorescence lifetime imaging microscopy (FLIM)-Förster resonance energy transfer (FRET) microscopy data acquired in live cells coexpressing H2B-eGFP and H2B-mCherry. This multiplexed approach generates spatiotemporal maps of nuclear-wide chromatin compaction that, when coupled with laser microirradiation-induced DSBs, quantify the size, stability, and spacing between compact chromatin foci throughout the DDR. Using this technology, we identify that ataxia–telangiectasia mutated (ATM) and RNF8 regulate rapid chromatin decompaction at DSBs and formation of compact chromatin foci surrounding the repair locus. This chromatin architecture serves to demarcate the repair locus from the surrounding nuclear environment and modulate 53BP1 mobility.
Saccharomyces cerevisiae has the most comprehensively
characterized protein–protein interaction network, or interactome,
of any eukaryote. This has predominantly been generated through multiple,
systematic studies of protein–protein interactions by two-hybrid
techniques and of affinity-purified protein complexes. A pressing
question is to understand how large-scale cross-linking mass spectrometry
(XL-MS) can confirm and extend this interactome. Here, intact yeast
nuclei were subject to cross-linking with disuccinimidyl sulfoxide
(DSSO) and analyzed using hybrid MS2-MS3 methods. XlinkX identified
a total of 2,052 unique residue pair cross-links at 1% FDR. Intraprotein
cross-links were found to provide extensive structural constraint
data, with almost all intralinks that mapped to known structures and
slightly fewer of those mapping to homology models being within 30
Å. Intralinks provided structural information for a further 366
proteins. A method for optimizing interprotein cross-link score cut-offs
was developed, through use of extensive known yeast interactions.
Its application led to a high confidence, yeast nuclear interactome.
Strikingly, almost half of the interactions were not previously detected
by two-hybrid or AP-MS techniques. Multiple lines of evidence existed
for many such interactions, whether through literature or ortholog
interaction data, through multiple unique interlinks between proteins,
and/or through replicates. We conclude that XL-MS is a powerful means
to measure interactions, that complements two-hybrid and affinity-purification
techniques.
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