HIGHLIGHTSEthanol as the only organic product obtained from highperformance CO 2 hydrogenation Key step for C-C bond is achieved between CO 2 and surface methyl at Cu step sites Current ethanol synthesis from CO 2 hydrogenation should be useful in industry Assemblies of surroundings and reactive centers are important for novel catalysts

Discrete nanosheets of silicon‐doped AlPO4 molecular sieves (SAPO‐34) with a thickness of ≈7 nm have been prepared through morphology‐reserved synthesis with a lamellar aluminum phosphate as precursor. Cages of the nanosheets are in situ incorporated with copper oxide clusters. The CuO@SAPO‐34 nanosheets exhibit a large external surface area with a high number of (010) channel pores on the surface. Due to the thin morphology, copper oxide clusters occupy the outmost cages with a probability >50 %. The distinctive configuration facilitates a new concept of pore mouth catalysis, i.e., reactant molecules larger than the pores cannot enter the interior of the molecular sieves but can interact with the CuO clusters at “the mouth” of the pore. In heterogeneous catalysis, CuO@SAPO‐34 nanosheets have shown top performance in one‐pot oxidation of cyclohexane to adipic acid by O2, a key compound for the manufacture of nylon‐66, which is so far produced using non‐green nitric acid oxidation.

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Cerebrospinal fluid (CSF) is an important sample source for diagnosing diseases in the central nervous system (CNS), but collecting and injecting CSF in small animals is technically challenging and often results in high mortality rates. Here, we present a cost-effective and efficient method for accessing the CSF in live rodents for fluid collection and infusion purposes. The key element of this protocol is a metal needle tool bent at a unique angle and length, allowing the successful access of the CSF through the foramen magnum. With this method, we can collect 5–10 µL of the CSF from mice and 70–100 µL from rats for downstream analyses, including mass spectrometry. Moreover, our minimally-invasive procedure enables iterative CSF collection from the same animal every few days, representing a significant improvement over prior protocols. Additionally, our method can be used to inject solutions into mice cisterna magna with high success rates and high postoperative recovery rates. In summary, we provide an efficient and minimally-invasive protocol for collecting and infusing reagents into the CSF in live rodents. We envision this protocol will facilitate biomarker discovery and drug development for diseases in the central nervous system.

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