The Drosophila Nanos protein is a localized repressor of hunchback mRNA translation in the early embryo, and is required for the establishment of the anterior-posterior body axis. Analysis of nanos mutants reveals that a small, evolutionarily conserved, C-terminal region is essential for Nanos function in vivo, while no other single portion of the Nanos protein is absolutely required. Within the C-terminal region are two unusual Cys-Cys-His-Cys (CCHC) motifs that are potential zinc-binding sites. Using absorption spectroscopy and NMR we demonstrate that the CCHC motifs each bind one equivalent of zinc with high affinity. nanos mutations disrupting metal binding at either of these two sites in vitro abolish Nanos translational repression activity in vivo. We show that full-length and C-terminal Nanos proteins bind to RNA in vitro with high affinity, but with little sequence specificity. Mutations affecting the hunchback mRNA target sites for Nanos-dependent translational repression were found to disrupt translational repression in vivo, but had little effect on Nanos RNA binding in vitro. Thus, the Nanos zinc domain does not specifically recognize target hunchback RNA sequences, but might interact with RNA in the context of a larger ribonucleoprotein complex.

This paper introduces the Flexible Global Ocean‐Atmosphere‐Land System Model: Grid‐Point Version 3 (FGOALS‐g3) and evaluates its basic performance based on some of its participation in the sixth phase of the Coupled Model Intercomparison Project (CMIP6) experiments. Our results show that many significant improvements have been achieved by FGOALS‐g3 in terms of climatological mean states, variabilities, and long‐term trends. For example, FGOALS‐g3 has a small (−0.015°C/100 yr) climate drift in 700‐yr preindustrial control (piControl) runs and smaller biases in climatological mean variables, such as the land/sea surface temperatures (SSTs) and seasonal soil moisture cycle, compared with its previous version FGOALS‐g2 during the historical period. The characteristics of climate variabilities, for example, Madden‐Julian oscillation (MJO) eastward/westward propagation ratios, spatial patterns of interannual variability of tropical SST anomalies, and relationship between the East Asian Summer Monsoon and El Niño–Southern Oscillation (ENSO), are well captured by FGOALS‐g3. In particular, the cooling trend of globally averaged surface temperature during 1940–1970, which is a challenge for most CMIP3 and CMIP5 models, is well reproduced by FGOALS‐g3 in historical runs. In addition to the external forcing factors recommended by CMIP6, anthropogenic groundwater forcing from 1965 to 2014 was incorporated into the FGOALS‐g3 historical runs.

Using the European Centre for Medium‐Range Weather Forecasts Interim Reanalysis data set and the tropical cyclone (TC) best track data obtained from the International Best Tracks Archive for Climate Stewardship project from June to November for the epoch of 1979–2013, this study reexamines the recent interdecadal shift in TC genesis over the western North Pacific (WNP) in the late 1990s. An interdecadal decrease in the frequency of the WNP TC genesis was observed during 1998–2013 compared to the period of 1979–1997. The spatial distribution of the interdecadal decrease of the WNP TC genesis running northwest‐southeast, consistent with the monsoon trough (MT) zone. The results imply that the WNP genesis may be closely related to the MT on the interdecadal timescale. After the late 1990s, the intensity of the WNP MT becomes weaker and it extends more westward, thereby providing an unfavourable condition for the TC genesis. Through a diagnosis of the energetics, we suggested that the barotropic energy conversion in the eastern part of the WNP MT tends to be weakened in the late 1990s, hence less energy to support the WNP TC genesis. Both the meridional shear and the convergence of the mean zonal winds over the eastern WNP MT have an important impact on the conversion from mean kinetic energy to eddy kinetic energy, which can be considered as the atmospheric cause of the interdecadal decrease of TC genesis.