The efficacy of anaesthetic tricaine methanesulfonate (MS-222) was evaluated in four freshwater aquarium fish species, Zebrafish (Danio rerio), Guppy (Poecilia reticulata), Discu (Symphysodon discus) and Green swordtail (Xiphophorus helleri).The correct dose of anaesthetic should induce the plane 4 of anaesthesia in less than 180 s, recovery in less than 300 s and must survive when exposed during 30 min to anaesthetic. Fishes were exposed to six concentrations of anaesthetic (75, 100, 125, 150, 200 and 250 mg L À1 ) and the time of fish reaching plane 4 of anaesthesia, post exposure recovery, and the percentage of survival when fish were subject to 30 min in the anaesthetic were recorded. The optimal doses varied according to the species: D. rerio -75, 100 and 125 mg L À1 , P. reticulata -125, 150 and 200 mg L À1 , S. discus -75 and 100 mg L À1 and X. helleri -125 and 150 mg L À1 . The induction time generally decreased significantly with increasing concentration of MS-222 for all of the species evaluated. The recovery time had a tendency to increase with the increase of the MS-222 concentration for D. rerio, P. reticulata and S. discus. On the other hand, X. helleri recovery time decreased with the increase of MS-222 concentration. MS-222 proved to be effective in anaesthesia for all the freshwater ornamental species studied. The main results clearly show that the optimal dose to anesthetize is fish species dependent and it is completely wrong to extrapolate optimal anaesthetic concentrations between different species.
The emergence of millimeter-wave based technologies is pushing the deployment of the 5th generation of mobile communications (5G), on the potential to achieve multi-gigabit and low-latency wireless links. Part of this breakthrough was only possible with the introduction of small antenna arrays, capable to form highly directional and electronically steerable beams. This strategy allowed the overcoming of some drawbacks, but with a higher price: the re-design of the lower layers by introducing beamforming techniques. The impact of these changes is not well studied on the higher layers, in the most recent stacks (IEEE 802.11ad, 3GPP). Thus, the study of real deployments and the use of accurate network simulators play a key role, by enabling the test of complex large-scale scenarios. This article presents a key component missing in the simulation of mmWave networks, a blockage model. To the best of our knowledge, this is first blockage model that emulates the effects of obstacles in the mmWave links. Additionally, a codebook generation of a phased antenna array, with the Quasi-Deterministic (Q-D) channel model is also presented. All models are tested and compared with an outdoor mmWave network using the IEEE 802.11ad standard. The simulated and the real-life tests show similar results, with an average error for the worst case of 2.43% (index ranges) and 4.51% (distance), and to an average standard deviation of about 1.33 dBm and 2.26 dBm.
The wireless backhaul has become a key enabler for 5G technology by presenting a costeffective and scalable alternative to the typical fiber backhaul. WiGig protocols, such as IEEE 802.11ad and later IEEE 802.11ay, have been considered for backhaul connectivity of 5G mobile networks, thanks to the availability of high bandwidths capable of achieving fiber-like data rates. However, this band suffers from high propagation loss that can only be compensated using highly directional antennas, making mmWave links more susceptible to blockage and errors. Thus, to effectively evaluate the viability of WiGig-based technologies in wireless backhaul scenarios, it is crucial to characterize the impact of obstruction across the different network layers. This article presents an extensive measurement campaign and cross-layer analysis of physical (PHY), medium access control (MAC), and transport layers metrics measured for outdoor WiGig-based hardware submitted to short-term and long-term blockage. This study found that maintaining constant and higher modulation and coding schemes (MCSs) in long-term blocked channels may induce packet errors as high as 100%, round-trip-time (RTTs) that can be in the order of a few seconds, and packet losses as high as 90%. Even dynamically adjusting the MCS, the performance can be highly degraded. This effect was exacerbated in short-term links, as they suffered from more extreme MCS changes upon sudden obstructions. Temporary line of sight (LOS) obstruction was shown to cause maximum delays of half a second and a PER of around 20%; in more extreme cases, it has even led to temporary link failures.
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