The northern bald ibis Geronticus eremita was once widespread throughout the Middle East, northern Africa, and southern and central Europe. Habitat destruction, persecution and the impacts of pesticides have led to its disappearance from most of its former range. It disappeared from central Europe > 400 years ago, but has persisted as a relict and slowly growing breeding population in Morocco, where c. 700 wild birds of all ages remain. In Algeria, the last confirmed breeding was in 1984; in Turkey the fully wild population disappeared in 1989, but a population remains in semi-wild conditions. In Syria a small population was rediscovered in 2002, only to subsequently decline to functional extinction. Restoration programmes have been initiated independently in several locations, with over 300 free-flying birds resulting from reintroduction projects in Austria, Germany, Spain and Turkey, to restore both sedentary and fully migratory populations. Maintaining current efforts in Morocco remains a high conservation priority.
Summary:The objective of this experiment was to evaluate spermatological parameters and fertilizing ability of short term stored semen in different extenders from endemic Abant trout (Salmo trutta abanticus T,1954). Semen was collected from 30 adult males by the hand stripping method without anesthesia. Having determined the main spermatological properties (volume, motility, movement duration, concentration and pH), the pooled samples were diluted at a 1:2 ratio with two extenders (0.3 M glucose or Ringer solution). The diluted semen was stored for 48 hours at 4°C. Following the cooled storage, motility of the spermatozoa was evaluated after 24 and 48 hours regarding the post-cooled period. Dry fertilization technique was used for the fertilization trials. Eggs were pooled from 30 females by the hand stripping method. Fertilization took place in dry plastic dishes and 600 eggs were used in each fertilization trial. The sperm-egg ratio was approximately 0.25x10 6 sperm/egg. After swelling, eggs were rinsed with hatchery water (7 o C) and batches were placed into vertical incubation trays. The experimental success was assessed from sperm motility and the percent of eyed-egg 25 days after fertilization. Average fresh semen motility was 81.4 %. According to the results of the experiment, the highest motility (67 % after 24 h, 53 % after 48 h) and movement duration (60 s after 24 h, 42 s after 48 h) were determined by using glucose extender after 24 and 48 hours storage. Fertility rate of fresh semen was 84.4 %. The highest eyed-egg rate (80.4 %) was obtained from semen stored with glucose based extender after 24 h storage. After 48 hour storage fertilization yield for the semen diluted with 0.3 M Glucose decreased to 61.9 % and to 43.8 % for the one diluted with Ringer solution. Our results indicate that glucose based extender is a better preservative than Ringer solution for the short term preservation of Abant trout semen.Key words: Abant trout, semen, short-term preservation, fertilityKısa süreli saklanmış Abant alabalığı spermatozoonlarının fertilizasyon yeteneği Özet: Bu araştırma ile, ülkemize ait endemik bir balık türü olan Abant alabalığının spermatolojik parametrelerinin değerlendirilmesi ve farklı sulandırıcılarda kısa süreli saklanan spermatozoonların fertilizasyon yeteneğinin ortaya konulması amaçlanmıştır. Bu araştırmada, 30 ergin erkek balıktan anestezi yapılmaksızın masaj yoluyla sperma alınmıştır. Birincil spermatolojik parametreler (miktar, motilite, haraket süresi, yoğunluk ve pH) belirlendikten sonra birleştirilen örnekler iki farklı sulandırıcı ile (0,3 M glukoz veya Ringer solusyonu) 1:2 (sperma:sulandırıcı) oranında sulandırılmıştır. Sulandırılan sperma 4°C'de 48 saat saklanmıştır. Saklama işlemi esnasında 24. ve 48. saatlerde motilite değerlendirmesi yapılmıştır. Fertilizasyon denemeleri için kuru fertilizasyon tekniği kullanılmıştır. Yumurtalar 30 ergin dişiden masaj yoluyla alınmıştır. Fertilizasyon kuru plastic kaplarda gerçekleştirilmiş ve her bir fertilizasyon denemesi için 600 yumurta ku...
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