Enteroaggregative Escherichia coli (EAEC) from the O104:H4 specific serotype caused a large outbreak of bloody diarrhea with some complicated cases of hemolytic-uremic syndrome (HUS) in Europe in 2011. The outbreak strain consisted in an EAEC capable to produce the Shiga toxin (Stx) subtype 2a, a characteristic from enterohemorrhagic E. coli. QseBC two-component system detects AI-3/Epi/NE and mediates the chemical signaling between pathogen and mammalian host. This system coordinates a cascade of virulence genes expression in important human enteropathogens. The blocking of QseC of EAEC C227-11 (Stx+) strain by N-phenyl-4-{[(phenylamino) thioxomethyl]amino}-benzenesulfonamide (also known as LED209) in vivo demonstrated a lower efficiency of colonization. The periplasmic protein VisP, which is related to survival mechanisms in a colitis model of infection, bacterial membrane maintenance, and stress resistance, here presented high levels of expression during the initial infection within the host. Under acid stress conditions, visP expression levels were differentiated in an Stx-dependent way. Together, these results emphasize the important role of VisP and the histidine kinase sensor QseC in the C227-11 (Stx+) outbreak strain for the establishment of the infectious niche process in the C57BL/6 mouse model and of LED209 as a promising antivirulence drug strategy against these enteric pathogens.
IMPORTANCE EAEC is a remarkable etiologic agent of acute and persistent diarrhea worldwide. The isolates harbor specific subsets of virulence genes and their pathogenesis needs to be better understood. Chemical signaling via histidine kinase sensor QseC has been shown as a potential target to elucidate the orchestration of the regulatory cascade of virulence factors.
Helicobacter pylori inhabits the
gastric epithelium and can promote the development of gastric disorders,
such as peptic ulcers, acute and chronic gastritis, mucosal lymphoid
tissue (MALT), and gastric adenocarcinomas. To use nanotechnology
as a tool to increase the antibacterial activity of silver I [Ag(I)]
compounds, this study suggests a new strategy for H.
pylori infections, which have hitherto been difficult
to control. [Ag (PhTSC·HCl)2] (NO3)·H2O (compound 1) was synthesized, characterized, and loaded
into polymeric nanoparticles (PN1). PN1 had been developed by nanoprecipitation
with poly(ε-caprolactone) polymer and poloxamer 407 surfactant.
System characterization assays showed that the PNs had adequate particle
sizes and ζ-potentials. Transmission electron microscopy confirmed
the formation of polymeric nanoparticles (PNs). Compound 1 had a minimum
inhibitory concentration for H. pylori of 3.90 μg/mL, which was potentiated to 0.781 μg/mL
after loading. The minimum bactericidal concentration of 7.81 μg/mL
was potentiated 5-fold to 1.56 μg/mL in PN. Compound 1 loaded
in PN1 displayed better activity for H. pylori biofilm formation and mature biofilm. PN1 reduced the toxicity of
compound 1 to MRC-5 cells. Loading compound 1 into PN1 inhibited the
mutagenicity of the free compound. In vivo, the system
allowed survival of Galleria mellonella larvae at a concentration of 200 μg/mL. This is the first
demonstration of the antibacterial activity of a silver complex enclosed
in polymeric nanoparticles against H. pylori.
Background
The intestinal microbiota plays a crucial role in human health, adjusting its composition and the microbial metabolites protects the gut against invading microorganisms. Enteroaggregative E. coli (EAEC) is an important diarrheagenic pathogen, which may cause acute or persistent diarrhea (≥14 days). The outbreak strain has the potent Shiga toxin, forms a dense biofilm and communicate via QseBC two-component system regulating the expression of many important virulence factors.
Results
Herein, we investigated the QseC histidine sensor kinase role in the microbiota shift during O104:H4 C227–11 infection in the colonic model SHIME® (Simulator of the Human Intestinal Microbial Ecosystem) and in vivo mice model. The microbiota imbalance caused by C227–11 infection affected ỿ-Proteobacteria and Lactobacillus spp. predominance, with direct alteration in intestinal metabolites driven by microbiota change, such as Short-chain fatty acids (SCFA). However, in the absence of QseC sensor kinase, the microbiota recovery was delayed on day 3 p.i., with change in the intestinal production of SCFA, like an increase in acetate production. The higher predominance of Lactobacillus spp. in the microbiota and significant augmented qseC gene expression levels were also observed during C227–11 mice infection upon intestinal depletion. Novel insights during pathogenic bacteria infection with the intestinal microbiota were observed. The QseC kinase sensor seems to have a role in the microbiota shift during the infectious process by Shiga toxin-producing EAEC C227–11.
Conclusions
The QseC role in C227–11 infection helps to unravel the intestine microbiota modulation and its metabolites during SHIME® and in vivo models, besides they contribute to elucidate bacterial intestinal pathogenesis and the microbiota relationships.
Invasive non-typhoidal Salmonella (iNTS) from the clonal type ST313 (S. Typhimurium ST313) is the major cause of invasive salmonellosis disease in Africa. Recently in Brazil, iNTS ST313 strains have been isolated from different sources, but there is a lack of understanding the mechanisms behind how these gut bacteria are able to break the gut barrier and reach the patient’s bloodstream. Herein, we compared 13 S. Typhimurium ST313 strains genomes isolated from human-blood cultures investigating aspects of virulence and resistance mechanisms. RNAseq analyses were also performed between the clinical blood isolate and SL1344 prototype, which belongs to ST19 and it was originally isolated from human feces. That analysis reveals here 15-upregulated genes related to pathogenesis in S. Typhimurium ST313 compared to SL1344 (ST19) such as sopD2, sifB, pipB, amongst others. We have also compared these clinical with non-clinical isolates from Brazil, a total of 22 genomes were studied by single nucleotide polymorphism (SNPs). The epidemiological analysis of 22 genomes of S. Typhimurium ST313 strains grouped them into three distinct clusters (A, B and C) by SNP analysis, where cluster A comprised five, the group B six, and the group C 11. The 13 clinical blood isolates were all resistant to streptomycin, 92. 3% strains were resistant to ampicillin and 15.39% strains were resistant to kanamycin. The resistance genes acrA, acrB, mdtK, emrB, emrR, mdsA and mdsB related to the production of efflux pumps were detected in all (100%) strains studied, similar to pathogenic traits investigated. In conclusion, we evidenced the S. Typhimurium ST313 strains isolated in Brazil are different of the African strains ST313. The elevated frequencies of virulence genes such as sseJ, sopD2 and pipB are a major concern in these Brazilian isolates, showing a higher pathogenic potential.
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