Various kinds of sequential polypeptides were prepared by direct polymerization of peptides in the presence of diphenylphosphoryl azide (DPPA). Typically, polymerization reactions were carried out as follows; monomer peptide concentration: 300 mg/0,6 mL of DMSO (or some other solvent); amount of DPPA: 1,3 mol/mol peptide; amount of triethylamine: 2,3 mol/mol peptide (+1,0 mol/mol peptide in case of peptide hydrochloride); reaction time: 2 days at room temperature. Molecular weights of the resulting sequential polypeptides were estimated from their intrinsic viscosities in dichloroacetic acid and SDS-polyacrylamide gel electrophoresis; they range from 4000 to 30000. The purity of the sequential polypeptides was confirmed by IR, elemental analysis and optical rotation measurements of their acid total hydrolysates.
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