The difference in reaction conditions between the syntheses of mono-and diacylglycerols (MAG and DAG) was elucidated in terms of the enzymatic glycerolysis of high-oleic sunflower oil containing 89% oleic acid. The most efficient lipases were Pseudomonas lipoprotein lipase and the lipase from Pseudomonas cepacia for the MAG and DAG syntheses, respectively. In each case, the glycerol amount to be added to maximize the yield was 1.5-fold larger than the stoichiometric amount that is necessary to complete the glycerolysis reaction. The addition of a small amount of acetone to the reaction mixture was only slightly effective on the yield of MAG. The control of the reaction temperature was very important, and the critical temperature, below which the yield of MAG or DAG is significantly increased, was found to be lower for DAG synthesis than for MAG synthesis. The reaction time that was required to obtain a maximum yield was about 80 h for MAG synthesis, while it was 6-fold longer for DAG synthesis. The content of MAG and monooleoylglycerol approached 90 and 80% in the lipid reaction products, respectively. On the other hand, the content of DAG was 82%, of which the fatty acid composition was similar to that of the original oil.Keywords: acetone, monooleoylglycerol, glycerolysis, high-oleic sunfiower oil, Iipase, monoacylglycerol, diacylglycerol A Iarge quantity of monoacylglycerols (MAG) is used as emulsifying agents in the manufacture of foods and cosmetics.