The odontoclast, which is morphologically similar to osteoclast, is thought to play a major role in root resorption of deciduous teeth. High collagenolytic activity has been detected in the root resorbing tissue. In order to identify collagenase-producing cells and the role of collagenase in root resorption of deciduous tooth, in situ hybridization of collagenase mRNA in bovine root-resorbing tissue sections was performed using a digoxigenin-labeled, nonradioactive RNA probe. Collagenase mRNA expression was clearly observed in odontoclasts in addition to the macrophages, fibroblasts, odontoblasts, and cementoblasts. Multinuclear odontoclasts showed intense tartrate-resistant acid phosphatase (TRAP) activity. We also examined interleukin-1 (IL-1) mRNA expression in the root-resorbing tissue by in situ hybridization. IL-1 transcripts were found to be expressed in odontoclasts, fibroblasts, and macrophages suggesting that IL-1 might be an important factor for promoting root resorption. These results suggest that the collagenase produced by odontoclasts may play a key role in dentin collagen degradation in root resorption.
The results of this study highlight the value of LAMC2 as a marker of cancer invasion. LAMC2-positive foci in leukoplakia suggest an imminent risk of cancer. LAMC2 immunostaining is expected to contribute to a more precise assessment of the malignancy of leukoplakia.
Necrotizing sialometaplasia is a rare lesion that mimics malignant neoplasm, which is characterized by salivary gland metaplasia, necrosis, and inflammation. We present a case of necrotizing sialometaplasia that presented as an ugly ulcer at the junction of the soft and hard palates; the lesion was clinically suspected to be a malignant lesion. A 62‐year‐old man was referred to our department with a complaint of severe pain during swallowing. A crater‐like ulcer lesion was observed extending from the maxillary tuberosity to the soft palate. Histopathological diagnosis of necrotizing sialometaplasia was confirmed on biopsy. This lesion was spontaneously relieved.
Abstract-Effects of eperisone, an antispasmodic in skeletal muscle, were inves tigated in helical strips of dog saphenous artery and vein. Eperisone relaxed saphenous arteries and veins previously contracted with norepinephrine, serotonin, acetylcholine, K+, or Bat+; but in contrast, it produced contractions in the blood vessels contracted with prostaglandin (PG) Fla. Treatment with eperisone at tenuated the contractions induced by norepinephrine and serotonin in the arteries and those by clonidine and phenylephrine in the veins. Eperisone inhibited angio tensin II-induced relaxations, mediated possibly by endogenous PG12, but did not alter relaxations caused by exogenous PGI2. Treatment with eperisone (10-5 M) potentiated the contractile response to electrical stimulation of adrenergic nerves; the potentiating effect was suppressed by yohimbine. The eperisone-induced contraction in PGF2a-contracted arteries was inhibited by treatment with indo methacin or aspirin, although cyclooxygenase activity was not inhibited by eperisone. These results may indicate that eperisone blocks postjunctional ai and a2 adren ergic, muscarinic, serotonergic receptors and prejunctional a2 adrenoceptors and reduces PGI2 synthesis via a mechanism other than cyclooxygenase inhibition.Eperisone hydrochloride (4'-ethyl-2-meth yl-3-piperidinopropiophenone hydrochloride) possesses antitremorine and antinicotinic actions (1) and inhibits mono and multi synaptic reflexes by suppressing a and r-efferent neuron activities in the spinal cord and supraspinal structures (2). The drug is clinically used to relieve skeletal muscle stif fness. Recent studies indicate an eperisone induced increase in blood flow in the skeletal muscle of anesthetized rabbits (personal com munication from Dr. M. Arai) and dogs (3).The agents possessing the piperidinopro piophenone structure relax visceral and vas cular smooth muscles (4-7), possibly due to a direct action on smooth muscle, like that of papaverine (6, 7). Fujioka and Kuriyama (8) have reported that eperisone has Ca2+ an tagonistic actions in isolated guinea pig basilar artery. Our preliminary study showed that eperisone did not relax but contracted the isolated dog saphenous artery and vein pre viously contracted with PGF2a• indicating that its action is different from those of papa verine and Ca2+ antagonists.Therefore, the present study was under taken to determine the action and the me chanisms of action of eperisone on receptor mediated responses of various agents in isolated saphenous arteries and veins, skeletal muscle vasculatures. Abilities of this drug to inhibit contractions mediated by a-adrenergic, serotonergic and muscarinic receptors and to potentiate contractions due to adrenergic nerve stimulation were demonstrated. Materials and MethodsMale and female mongrel dogs, weighing 7-13 kg, were anesthetized with intravenous injections of sodium pentobarbital (30 mg/kg) and killed by bleeding from the carotid ar teries. Saphenous arteries and veins and inter lobar branches of the renal artery ...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.