Gel filtration was used to partially purify the antioxidizing component of a centrifuged serum of raw cow milk, and its properties were studied. The antioxidizing effect did not decrease after dialysis or heating. The finding of no change after dialysis suggested that the compound was of high molecular weight, estimated at 38,000 by gel filtration. The antioxidizing effect decreased with irradiation, accompanied by a parallel decrease in the riboflavin content. The antioxidizing component of this serum fraction may be a protein-bound riboflavin.
Wurzenberger, M.; Gorsch, W. Enzymic oxidation of linolenic acid to l,Z-5-octadien-3-ol, Z-2-Z-5-octadien-l-ol and 10-oxo-E-8decenoic acid by a protein fraction from mushroom. (Psalliota bis pora). Lipids 1986, 21, 261-266.
Assay of antioxidizing actionWe studied the antioxidant effects of protein-bound riboflavin and free riboflavin using methyl linoleate in a water system in the dark. The protein-bound riboflavin inhibited the formation of hydroperoxides. After exposure to light, the protein lost its antioxidant effect, which suggested that it was not the protein-bound riboflavin but the riboflavin itself that had the antioxidant effect. These results showed that riboflavin inhibited the formation of hydroperoxides, which suggested that it did have an antioxidant effect.
The photolysis mechanism of riboflavin (RF) in milk serum was studied. Whenmilk serum was light-illuminated, the decomposition of RFin the serum was accelerated. Superoxide anion (O2T) and hydrogen peroxide (H2O2) were found to form during the illumination, while superoxide dismutase (SOD) activity could not be detected in the milk serum. The decomposition of RF was significantly inhibited by the addition of tryptophan or mannitol which are scavengers of the hydroxyl radical. Conversely, addition of Fe3+ or lactoferrin promoted the decomposition.These results strongly suggest the direct participation of the hydroxyl radical in the RF decomposition in milk serum during light-illumination. It was also suggested that the hydroxyl radical was formed via the iron catalyzed Haver-Weiss reaction in which iron reacts with O2 T and H2O2, although it is still unknown how O2T and H2O2were generated by illumination.
The size, shape and aggregate structure of blood cell particle from the universal experiment silkworm (STS-84 EDl/EDl) were investigated by using scanning electron microscopy (Natural SEM). Comparing with STS-84 EDl/EDl, the un-universal experiment silkworm (EDl/EDl) having heredity factor of EDl/EDl which grown up under the ordinary circumstance was used as a sample of reference.Blood cells of silkworm (STS-84 EDl/EDl and EDl/EDl) were based on two types of particle, one was round sphere particle and another type was fibrous particle. Some of these particles gathered and entangled. Two types of particle formed the network structure of various types of aggregates. The round sphere particle of STS-84 EDl/EDl was the smaller than EDl/EDl, and the fibrous particle of STS-84 EDl/EDl had higher density structure than EDl/EDl
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.