Since Gabrio et a1.1' reported that the addition of inosine could prolong the survival time of human red blood cells when stored in acid citrate-dextrose (ACD) solution at 4°C, cooperative studies2'3' have been carried out in which the posttransfusion survival of the red blood cells stored in ACD alone and ACD containing inosine (ACDI) was compared. On the basis of the results of biochemical studies on the phosphorus metabolism in human red blood cells, M. Nakao et al, have proposed a theory explaining the process of adenosine triphosphate (ATP) degradation encountered in the human red blood cells during preservation,4' and have reported briefly that the addition of adenine and inosine suppressed markedly not only the ATP degradation in red blood cells, but also the decrease in the glycolytic activity, the increase in osmotic fragility and the shape-change of the cells.5' This note is concerned with the results of studies on the posttransfusion survival of the human red blood cells which were preserved in ACD solution containing both adenine and inosine (ACDIA) for 3 weeks.Materials and methods. About 200 ml of whole blood was collected from a professional donor, divided into three portions and placed in sterile siliconized glass bottles containing ACD solution of one-fifth volume of the whole blood. Either one-tenth volume of the saline of adenine and inosine or inosine alone, or saline alone, was added to each bottle containing ACD blood. The final concentration of adenine and inosine in the bottle was 1.25 mMol/ l and 2.5 mMol/l, respectively.After preservation, 150 micro-curies of radioactive sodium chromate (Na2Cr5104) was added to every 50 ml of the whole blood, and allowed to stand at room temperature for 30 to 60 minutes with gentle mixing every five minutes. Then the whole blood was centrifuged at low speed to remove the supernatant, and red cells were washed once with the saline and resuspended in the saline to the original volume. With
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