To determine the effect of long-distance (approximately 600 km) road transportation on
the blood biochemistry of laboratory animals, we investigated the changes in serum
biochemical parameters in healthy cynomolgus monkeys and beagle dogs transported by truck
from Osaka to Tsukuba, Japan. The concentrations of serum cortisol, total bilirubin and
aspartate aminotransferase in monkeys increased during transportation. Serum cortisol and
total bilirubin levels in dogs also increased during transportation, but serum
triglyceride decreased. Serum parameter values in truck-transported monkeys and dogs
returned to baseline levels within two weeks following arrival. Taken together, these
results suggest that a two-week acclimation period is the minimum duration required for
adaptation following road transportation.
1 We investigated the eects of FR122047 (1-[(4,5-bis(4-methoxyphenyl)-2-thiazoyl)carbonyl]-4-methylpiperazine hydrochloride), a selective cyclo-oxygenase (COX)-1 inhibitor, in rat type II collagen-induced arthritis (CIA) and adjuvant-induced arthritis (AIA). 2 Using an ex vivo rat whole blood assay, FR122047 (0.032 ± 3.2 mg kg
71) inhibited COX-1-derived thromboxane (TX) B 2 production with ED 50 value of 0.059 mg kg 71 , indicating that it was orally active, but did not inhibit lipopolysaccharide-induced prostaglandin (PG) E 2 production derived by COX-2. 3 Oral administration of FR122047 showed a dose-dependent anti-in¯ammatory eect in rat CIA with ED 50 value of 0.56 mg kg
71. This drug also dose dependently suppressed the levels of PGE 2 and TXB 2 in CIA rat paws with ED 50 values of 0.24 and 0.13 mg kg
71, respectively. 4 FR122047 had no eect in rat AIA model. In contrast, indomethacin, a non-selective COX inhibitor, was anti-in¯ammatory and reduced the formation of PGs in AIA rat paws. 5 Unlike indomethacin, chronic treatment of FR122047 did not damage the stomach mucosa in CIA rats. 6 These results demonstrate that COX-1 contributes to the oedema and the formation of PGE 2 and TXB 2 in rat CIA model, but not in rat AIA model. 7 We conclude that FR122047 has an orally active and anti-in¯ammatory eect mediated by inhibition of PGE 2 and TXB 2 produced by COX-1 at a site of in¯ammation induced by type II collagen and it may be a useful tool for studying the involvement of COX-1 in various in vivo models of in¯ammation.
The anesthetic effect of a
combination of medetomidine, midazolam and butorphanol (Me-Mi-Bu) was evaluated in healthy
cynomolgus monkeys. The Me-Mi-Bu combination was intramuscularly administered as follows:
Dose 1, Me 0.015 mg/kg-Mi 0.1 mg/kg-Bu 0.15 mg/kg; Dose 2, Me 0.02 mg/kg-Mi 0.15 mg/kg-Bu
0.2 mg/kg; and Dose 3, Me 0.04 mg/kg-Mi 0.3 mg/kg-Bu 0.4 mg/kg. The combination rapidly
induced immobilization, and lateral recumbency was reached within 15 min. The duration of
anesthesia for each dose administered was follows: Dose 1, 47 ± 27 min; Dose 2, 113 ± 31
min; and Dose 3, 190 ± 24 min. The anesthetic effect of the combination was abolished by
the α2-adrenoceptor antagonist atipamezole. No marked changes in the levels of
hematologic or serum biochemical parameters were noted in cynomolgus monkeys administered
the combination plus atipamezole. Taken together, these results suggest that the Me-Mi-Bu
combination exhibits reversible anesthetic effect and may be useful for studies involving
cynomolgus monkeys.
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