Recently, a plasma flow has been applied to medical treatment using effects of various kinds of stimuli such as chemical species, charged particles, heat, light, shock wave and electric fields. Among them, the chemical species are known to cause an inactivation of cell viability. However, the mechanisms and key factors of this event are not yet clear. In this study, we focused on the effect of H2O2 in plasma-treated culture medium because it is generated in the culture medium and it is also chemically stable compared with free radicals generated by the plasma flow. To elucidate the significance of H2O2, we assessed the differences in the effects of plasma-treated medium and H2O2-added medium against inactivation of HeLa cell viability. These two media showed comparable effects on HeLa cells in terms of the survival ratios, morphological features of damage processes, permeations of H2O2 into the cells, response to H2O2 decomposition by catalase and comprehensive gene expression. The results supported that among chemical species generated in a plasma-treated culture medium, H2O2 is one of the main factors responsible for inactivation of HeLa cell viability.

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The Venusian atmosphere is in a state of superrotation where prevailing westward winds move much faster than the planet’s rotation. Venus is covered with thick clouds that extend from about 45 to 70 km altitude, but thermal radiation emitted from the lower atmosphere and the surface on the planet’s night-side escapes to space at narrow spectral windows of near-infrared. The radiation can be used to estimate winds by tracking the silhouettes of clouds in the lower and middle cloud regions below about 57 km in altitude. Estimates of wind speeds have ranged from 50 to 70 m/s at low- to mid-latitudes, either nearly constant across latitudes or with winds peaking at mid-latitudes. Here we report the detection of winds at low latitude exceeding 80 m/s using IR2 camera images from the Akatsuki orbiter taken during July and August 2016. The angular speed around the planetary rotation axis peaks near the equator, which we suggest is consistent with an equatorial jet, a feature that has not been observed previously in the Venusian atmosphere. The mechanism producing the jet remains unclear. Our observations reveal variability in the zonal flow in the lower and middle cloud region that may provide new challenges and clues to the dynamics of Venus’s atmospheric superrotation.

A mechanism for sterilizing Escherichia coli by a flowing postdischarge and UV radiation of argon plasma at atmospheric pressure was investigated by analyzing the surviving cells and the potassium leakage of cytoplasmic material and by morphological observation. Inactivation of E. coli results from the destruction of the cytoplasmic membrane and the outer membrane under plasma exposure and the destruction of nucleic acids by exposure to ultraviolet radiation from the plasma source.

The existence of large stationary gravity waves was discovered during Akatsuki's first observation sequence in 2015. In this study, the further detection of large stationary gravity waves in brightness temperature images over a 1.5 year period is reported. The waves periodically appeared mostly above four specific highland regions in the low latitudes when these regions were in the local afternoon. The wave amplitudes attenuated after the wave locations passed beyond the evening terminator, and the locations of the waves tended to slowly drift eastward over their lifetimes. The appearances of stationary waves depend not only on surface topography but also on latitude and local time, suggesting that solar heating during the daytime and atmospheric structure affected by solar heating may control the excitation and propagation of stationary waves. Plain Language Summary The Japanese Venus satellite "Akatsuki" has repeatedly found large atmospheric waves with north-south lengths, which sometimes reach more than 10,000 km at the cloud top level on Venus (altitude~70 km). These waves have repeatedly appeared above the Venusian highlands in low latitudes, such as Aphrodite Terra. Interestingly, the waves appeared and became clearer each time the highlands passed from noon to evening; therefore, they can be regarded as "daily" Venusian phenomena. Despite westward wind speeds reaching 100 m s À1 at the cloud top level (known as atmospheric superrotation), the east-west propagation speeds of the large waves were nearly zero, and the waves stayed above their initial locations (stationary). This means that the origin of the waves could be the highland terrains below. Because waves can transport energy via propagation, stationary waves may transport atmospheric energy from the lower atmosphere to the cloud top level and may affect the speed of the superrotation. The existence and regular appearance of the large stationary waves indicate a continuous interaction between the lower and upper atmospheres on Venus via wave propagation, which provides a novel perspective of the Venusian atmosphere.

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The aim of this study was to clarify the initiation process and the propagation mechanism of positive underwater streamers under the application of pulsed voltage with a duration of 10 μs, focusing on two different theories of electrical discharges in liquids: the bubble theory and the direct ionization theory. The initiation process, which is the time lag from the beginning of voltage application to streamer inception, was found to be related to the bubble theory. In this process, Joule heating resulted in the formation of a bubble cluster at the tip of a needle electrode. Streamer inception was observed from the tip of a protrusion on the surface of this bubble cluster, which acted as a virtual sharp electrode to enhance the local electric field to a level greater than 10 MV/cm. Streak imaging of secondary streamer propagation showed that luminescence preceded gas channel generation, suggesting a mechanism of direct ionization in water. Streak imaging of primary streamer propagation revealed intermittent propagation, synchronized with repetitive pulsed currents. Shadowgraph imaging of streamers synchronized with the light emission signal indicated the possibility of direct ionization in water for primary streamer propagation as well as for secondary streamer propagation.