: The recently developed technology of real-time cell analysis RTCA was designed to analyze cell migration and invasion in vitro. In this study, we investigated these cellular factors in oral squamous cell carcinomas OSCCs of the tongue and oor of the mouth with RTCA. We also examined the associated matrix metalloproteinases MMPs and integrins. We used the cell lines SCC-4 and SAS, which are human poorly differentiated OSCCs from the tongue, and HO-1-u-1, which are human poorly differentiated OSCCs from the oor of the mouth. Using RTCA, cell migration was assessed on fibronectin-coated CIM-Plates, and invasion was assessed on bronectin-and matrigel-coated CIM-Plates. SCC-4 cells demonstrated a high ability for cell migration and invasion compared with SAS and HO-1-u-1 cells. The SCC-4 cells also expressed high levels of MMP-9 and integrin 1 mRNA compared with SAS and HO-1-u-1 cells. The MMP inhibitor Marimastat blocked migration and invasion of all OSCCs. The ndings suggest that MMP-9 is associated with cell migration and invasion in OSCCs, and indicate that RTCA will be useful for analyzing the metastatic capability of OSCCs and developing more effective new drugs for this disease.
Patients diagnosed with obstructive sleep apnea syndrome OSAS between January and September in our hospital were examined for the presence of allergic rhinitis, and after excluding those with seasonal allergic rhinitis, patients were enrolled in the study. Examinations showed that % of OSAS patients also had allergic rhinitis, and comparison to those without allergic rhinitis revealed that there was a significant difference in the degree of nasal airflow. We also conducted polysomnography to compare the sleep conditions of patients. Patients with allergic rhinitis had significantly longer sleep latency and a slightly higher. These results showed that allergic rhinitis is associated with OSAS and might be a factor contributing to sleep disruption, thus exacerbating sleep conditions and the severity of OSAS in these patients. The results also indicate that treatment and improvement of allergic rhinitis and nasal obstruction will likely improve sleep apnea and the quality of sleep.
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