In studies of the functions of roots in the development of aboveground organs, the butanol fraction of xylem sap collected from squash root was found to have inhibitory activity against the formation of adventitious roots on the hypocotyls of cucumber in a culture of shoot cutting. The inhibitory activity was fracUonated with reverse phase column chromatographies, and an inhibitory fraction was recovered with a single peak of absorbance at 280 nm, which contained a novel amino acid, NS-
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