Over the past 20 y, many studies have examined the history of the plant ecological and molecular model, Arabidopsis thaliana, in Europe and North America. Although these studies informed us about the recent history of the species, the early history has remained elusive. In a large-scale genomic analysis of African A. thaliana, we sequenced the genomes of 78 modern and herbarium samples from Africa and analyzed these together with over 1,000 previously sequenced Eurasian samples. In striking contrast to expectations, we find that all African individuals sampled are native to this continent, including those from sub-Saharan Africa. Moreover, we show that Africa harbors the greatest variation and represents the deepest history in the A. thaliana lineage. Our results also reveal evidence that selfing, a major defining characteristic of the species, evolved in a single geographic region, best represented today within Africa. Demographic inference supports a model in which the ancestral A. thaliana population began to split by 120-90 kya, during the last interglacial and Abbassia pluvial, and Eurasian populations subsequently separated from one another at around 40 kya. This bears striking similarities to the patterns observed for diverse species, including humans, implying a key role for climatic events during interglacial and pluvial periods in shaping the histories and current distributions of a wide range of species.he plant Arabidopsis thaliana is the principal plant model species, and as such has been useful not only to examine basic biological mechanisms but also to elucidate evolutionary processes. The exceptional resources available in this species, including seed stocks collected from throughout Eurasia for over 75 y, have been a valuable tool for learning about the natural history of A. thaliana on this continent (1, 2). Previous studies have shown that current variation in Eurasia is mainly a result of expansions and mixing from refugia in Iberia, Central Asia, and Italy/Balkans after the end of the last glacial period ∼10 kya (3-8). The main finding of the recent analysis of 1,135 sequenced genomes was that a few Eurasian samples represent divergent relict lineages, whereas the vast majority derived from the recent expansion of a single clade (4). Given the large number of studies that examine the natural history of A. thaliana, one would expect that this history would by now be described rather completely and there would be no major surprises left to uncover. However, there are still many open questions about the ancient history of the species.Several features differentiate A. thaliana from its closest relatives. Although most members of the Arabidopsis genus are obligate outcrossing perennials with large flowers and genome sizes of over 230 Mb and 8 chromosomes, A. thaliana is a predominantly selfing annual with reduced floral morphology and a reduced genome size of ∼150 Mb and 5 chromosomes. The transition to predominant selfing in A. thaliana was likely the catalyst for these derived morphological and...
The Mla locus in barley (Hordeum vulgare) conditions isolate-specific immunity to the powdery mildew fungus (Blumeria graminis f. sp. hordei) and encodes intracellular coiled-coil (CC) domain, nucleotide-binding (NB) site, and leucine-rich repeat (LRR)-containing receptor proteins. Over the last decades, genetic studies in breeding material have identified a large number of functional resistance genes at the Mla locus. To study the structural and functional diversity of this locus at the molecular level, we isolated 23 candidate MLA cDNAs from barley accessions that were previously shown by genetic studies to harbor different Mla resistance specificities. Resistance activity was detected for 13 candidate MLA cDNAs in a transient gene-expression assay. Sequence alignment of the deduced MLA proteins improved secondary structure predictions, revealing four additional, previously overlooked LRR. Analysis of nucleotide diversity of the candidate and validated MLA cDNAs revealed 34 sites of positive selection. Recombination or gene conversion events were frequent in the first half of the gene but positive selection was also found when this region was excluded. The positively selected sites are all, except two, located in the LRR domain and cluster in predicted solvent-exposed residues of the repeats 7 to 15 and adjacent turns on the concave side of the predicted solenoid protein structure. This domain-restricted pattern of positively selected sites, together with the length conservation of individual LRR, suggests direct binding of effectors to MLA receptors.
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