No abstract

Potential risks of supply shortages for critical metals including rare-earth elements and yttrium (REY) have spurred great interest in commercial mining of deep-sea mineral resources. Deep-sea mud containing over 5,000 ppm total REY content was discovered in the western North Pacific Ocean near Minamitorishima Island, Japan, in 2013. This REY-rich mud has great potential as a rare-earth metal resource because of the enormous amount available and its advantageous mineralogical features. Here, we estimated the resource amount in REY-rich mud with Geographical Information System software and established a mineral processing procedure to greatly enhance its economic value. The resource amount was estimated to be 1.2 Mt of rare-earth oxide for the most promising area (105 km2 × 0–10 mbsf), which accounts for 62, 47, 32, and 56 years of annual global demand for Y, Eu, Tb, and Dy, respectively. Moreover, using a hydrocyclone separator enabled us to recover selectively biogenic calcium phosphate grains, which have high REY content (up to 22,000 ppm) and constitute the coarser domain in the grain-size distribution. The enormous resource amount and the effectiveness of the mineral processing are strong indicators that this new REY resource could be exploited in the near future.

Cytoskeleton-membrane-extracellular matrix interactions at the node of Ranvier were examined in both central and peripheral axons by combining three different methods for tissue preparation with three different electron microscopic techniques for imaging supramolecular structure. Conventional and three-dimensional high voltage electron microscopy of thin and semithick sections of tissues stained en bloc with ferric chloride revealed the presence of transcellular structures across the nodal gap traversing the paranodal glial-axonal junction. These structures penetrate both axonal and glial membranes and are further traced to the cortical axoplasm. This observation was verified by an examination of similar regions in rapidly-frozen freeze-substituted fresh axons. The filamentous nature of these structures, their focal attachment to the external true surface of the nodal and paranodal axolemma and their association with membrane particles were visualized in deep etch rotary-shadow replicas. At the node, both extracellular gap-crossing filaments and membrane-cytoskeletal linkers in the nodal axoplasm are joined to one of the prominent membrane particles of the nodal axolemma. At the paranodal axo-glial junction, the anchoring site of these membrane-cytoskeleton linkers are found on the linear arrays of 16 nm particles. Thus, cytoplasmic filaments and extracellular filaments or bridge structures are involved in the membrane-cytoskeletal interaction at the node and paranode. Some of these membrane particles are known to play a role in ionic conductances known to occur at this site. An additional role in cell adhesion or maintenance of the membrane specialization of this functionally important site of axolemma is now indicated.

The apical cytoplasm of several absorbing epithelia contains well-developed apical tubules (AT) which contribute to membrane recycling from endocytic vacuoles to the apical cell membrane. In this study, we examined three-dimensional structures of the AT in rat kidney proximal tubule cells by transmission and scanning electron microscopy. In thin sections, the AT appeared as straight tubules with a rather constant diameter (70-90 nm), but others were curved and, occasionally, branching. No AT were labeled with the marker for the external cell surface (ruthenium red) or exhibited histochemical enzyme activity for lysosomal hydrolase (acid phosphatase). After intravenous injection of horseradish peroxidase, it was absorbed in the kidney proximal tubule cells and the AT were labeled with HRP reaction products. Stereo-viewing of the labeled AT in thick sections revealed that they formed an interconnected tubular network. Scanning electron microscopy allowed a three-dimensional view of the AT, in which a network of branching and anastomosing tubules was revealed. These observations indicate that the AT are intracellular endosomal compartments which form an extensive tubular network in the apical cytoplasm. The possibility that this apical tubular network serves as a large membrane store for membrane recycling is discussed.