The ethanol production performance of the flocculating yeast S. cerevisiae ATCC26602 from non-sterilized kitchen refuse medium in repeated-batch culturing was investigated. Although no pH modification or asepsis technology was applied, no contamination occurred. Twenty cycles of repeated-batch fermentation over a period of 11 days were successfully carried out without any loss of productivity by the self-flocculating strain ATCC26602. In addition, from the third batch, the flocculent strain achieved the reduction of the fermentation time to half, which resulted in an increasing of ethanol productivity to 3.7 g/L/h. These results of ethanol fermentation from kitchen refuse under the non-sterilized condition by this acid-tolerant and self-flocculation yeast may pave the way to low-cost ethanol production in pilot applications.
The possibility of generating renewable bio-fuel energy through ethanol fermentation of kitchen refuse by Saccharomyces cerevisiae ATCC 26602 was established in this work. The acid-tolerant yeast S. cerevisiae ATCC 26602 was selected from among four S. cerevisiae strains as the best ethanol fermentation agent for kitchen refuse medium. The optimal kitchen refuse medium for ethanol production had a glucose concentration of only 12% (w/v) and consisted of saccharified kitchen refuse. Additional nitrogen supplementation was not necessary. A maximal ethanol concentration of 59.38 g/L with a 0.50 ethanol yield (YP/S) was obtained with the optimal medium composition in flask culture under shaking and without pH control. No significant difference was observed in ethanol
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