No abstract

Yeasts were isolated and the alcohol genic features were tested for their direct use in the wine processing of jambolan. In addition, changes in the total phenolic compounds during the maceration-fermentation process were investigated. Yeasts were selected from the spontaneous fermentation of the jambolan pulp, and the feasibility and fermentative production of its alcohol was tested. Out of the group of yeasts selected, the one that stood out was subjected to DNA extraction and sequencing. The yeast was identified as Saccharomyces cerevisiae, and the fermentation tests came back as 80.6% and the ethanol production yield was 8.35%. The chemical composition of raw materials was analyzed by spectrophotometrics and high performance liquid chromatography (HPLC) methods. The overall results also indicated that the evolution during the maceration-fermentation process of phenolic compound concentrations was influenced by the varietal factor. The concentration of phenolic compounds increased 30%, while the concentration of tannins increased 27.4% in the final product.

There are various known extraction methods that can be applied to the quantification of phenolic compounds, and thus it is up to the researcher to perfect the process that best applies to the sample in question. Different extraction solvents (liquid-liquid extraction) were compared in order to assess their effectiveness for the analysis of total phenols in jambolan ( Syzygium cumini Lamark) pulp extract using the conventional Folin-Ciocalteau methodology. Three CCRDs (Central Composite Rotational Designs) were carried out to evaluate the factors influencing the extraction process conditions. The variables studied were the solvent concentration (ethanol and methanol, diluted in water) and the mixing time in the compound extraction process. Of the conditions studied, it was shown that a 60% ethanol in water extraction solvent with a mixing time of 10 minutes, in addition to extracting a greater concentration of phenolic compounds, showed the greatest cost benefit advantage, since ethanol is cheaper and less toxic than methanol.