Genipap (Genipa americana L., Rubiaceae ) is a native Brazilian species and can be used in the recovery of degraded forest areas or for food supply. In order for the species to reach its potential, production of high quality seedlings is essential. The objective of this study was to evaluate genipap seedlings in protected environments and different substrates. The environments tested were: (1) a greenhouse with polyethylene film in the top, with aluminized screen (Aliminet ® ) of 50%-shading under this film, and lateral sides covered with 50%-shading nylon net (Sombrite ® ), (2) a shaded hut, all sides covered with 50%-shading nylon net (Sombrite ® ), and (3) a nursery shelter, with all lateral sides uncovered and the roof covered with leaves of buriti (Mauritia flexuosa). In these environments the following substrates were tested: 50% cattle manure + 50% cassava foliage, 50% cattle manure + 50% Vida Verde ® , 50% cattle manure + 50% vermiculite, and 25% cattle manure + 25% vermiculite + 25% of cassava foliage + 25% Vida Verde ® . Because there was no repetition of the growth environment, the effect of environment was examined using statistical procedures for analysis of combined experiments. Within environments a completely randomized design was used with five replications. All substrates are suitable for the formation of genipap seedlings, where the recommended substrates are: 50% cattle manure + 50% cassava foliage and 50% cattle manure + 50% Vida Verde ® for the greenhouse and the substrates composed of 50% cattle manure + 50% vermiculite and 25% cattle manure + 25% cassava foliage + 25% Vida Verde ® + 25% vermiculite for the shaded hut. The buriti shelter is not recommended for production of genipap seedlings. KEYWORDS: Genipa americana, cattle manure, cassava foliage, analysis of combined experiments.Ambientes protegidos e substratos para formação de mudas de jenipapeiro RESUMO O jenipapo (Genipa americana L., Rubiaceae) é uma espécie brasileira nativa e pode ser utilizada na recomposição de áreas de florestas degradadas ou fornecimento de alimentos. Para que a espécie atinja elevado potencial, a produção de mudas de elevada qualidade é essencial. O objetivo deste estudo foi avaliar mudas de jenipapo em ambientes protegidos e diferentes substratos. Os ambientes testados foram; (1) uma estufa agrícola com filme de polietileno transparente na cobertura, com tela aluminizada (Aliminet ® ) de 50% de sombreamento sob este filme, e lateral com tela de 50% de sombreamento; (2) uma cabana sombreada com todos os lados cobertos com tela de nylon de 50% de sombreamento (Sombrite ® ), e (3) um abrigo com todas as laterais sem fechamentos e cobertura com folhas de buriti (Mauritia flexuosa). Nestes ambientes foram testados os substratos 50% esterco bovino + 50% rama de mandioca, 50% esterco bovino + 50% Vida Verde ® , 50% esterco bovino + 50% vermiculita e 25% esterco bovino + 25% vermiculita + 25% rama de mandioca + 25% Vida Verde ® . Por não haver repetição do ambiente de cultivo, o efeito do ambiente foi ...