In enzyme-based biosensors, Ag + eluted from the reference electrode inhibits the enzyme activity. Herein, to suppress the inhibition of bilirubin oxidase (BOD) by Ag + , kinetic analysis was used to examine the effect of Ag + on the activity of BOD. It was confirmed that the addition of Ag + decreased the bioelectrocatalytic activity of BOD. Atomic absorption spectroscopy (AAS) suggested that Ag + was attached to BOD. Moreover, the changes in the visible absorption spectra after Ag + addition showed that Ag + was bound to the type I Cu sites in BOD. During oxygen reduction by BOD, the direct-electron-transfer-type bioelectrocatalytic current decreased after Ag + was added. The decay of the catalytic current was evaluated using kinetic analysis (assuming a pseudo-first-order reaction). Based on the analysis, the inhibition of BOD was suppressed when the Ag + concentration was below 0.1 µM. Referring to the solubility product of AgCl, Cl − at a concentration of 1 mM suppressed the inhibition of the enzymatic activity by 95%. KeywordsRedox enzyme • Type I copper • Oxygen reduction • Amperometric biosensor • Silver • Silver-chloride electrode * Yuki Kitazumi
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