We investigated the role of long non-coding RNA DLEU1 (deleted in lymphocytic leukemia 1) in the progression of papillary thyroid carcinoma (PTC). DLEU1 levels were higher in PTC cell lines (BHP5-16, TPC-1,8505C, and SW1736) and patient tissues (n=54) than in a human thyroid follicular epithelial cell line (Nthy-ori3-1) or adjacent normal thyroid tissues. High DLEU1 expression correlated positively with lymph node metastasis and advanced clinical stages in PTC patients. Bioinformatics, dual luciferase reporter, and RNA pulldown assays confirmed that DLEU1 directly binds to miR-421. Moreover, bioinformatics and dual luciferase reporter assays showed that miR-421 directly binds to the 3’untranslated region of the rho-related coiled-coil kinase 1 (ROCK1) in TPC-1 cells. PTC patient tissues and cell lines showed high ROCK1 mRNA and protein levels as well as low miR-421 levels. CCK-8, flow cytometry, wound healing, and Transwell invasion assays demonstrated that DLEU1 silencing decreases TPC-1 cell proliferation, survival and progression, but they can be rescued by miR-421 knockdown or ROCK1 overexpression. DLEU1 knockdown in TPC-1 cells decreased in vivo xenograft tumor size and weight compared to controls in nude mice. These findings demonstrate that DLEU1 promotes PTC progression by sponging miR-421 and increasing ROCK1 expression.
This work was developed to explore the adoption value of bacterial cellulose (BC) and heparin (He) combined with novel nano bio-vascular stents in carotid artery stent implantation, as well as the nursing methods and effects during the perioperative period. Based on the BC artificial blood vessel preparation method, the BC-He novel nano bio-vascular stent was fabricated after the modification of the medium method. Infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray electron diffraction (XRD) were performed to analyze the crystal structure and chemical structure of the material. In addition, scanning electron microscope (SEM) and transmission electron microscope (TEM) were employed to observe the micro-morphology of the stent. The mechanical properties and thermal stability of the materials were also characterized. Then, with 98 patients undergoing carotid artery stenting (CAS) as the research object, BC-He novel nano bio-vascular stent was applied to treat patients with arterial stenosis. According to different nursing methods, patients were assigned to the conventional nursing group (group A) and perioperative nursing group (group B). The postoperative complications, nursing effect, and satisfaction degree of the two groups were compared. It turned out that the BC-He novel nano bio-vascular stent was a kind of micro-mesh structure. There were characteristic absorption peaks of BC and He in BC-He, and there were N and S from He. Moreover, it also contained BC and He characteristic diffraction peaks, its tensile strength was significantly lower than BC, and its thermal stability was higher than BC. The preoperative carotid artery stenosis ranged from 50 % to 75 %, and the postoperative stenosis rate of restenosis patients treated with BC-He novel nano bio-vascular stent was less than 10 %. In addition, there were 92 patients (93.88 %) whose symptoms improved or markedly improved within 1 to 2 weeks after the operation, and 78 patients (79.6 %) had improved hemodynamic parameters. The incidence of postoperative complications in group A was dramatically superior to that in group B (P < 0.05). The total effective rate of nursing care and the degree of nursing satisfaction in group B were better than those in group A (P < 0.05). In short, BC-He novel nano bio-vascular stent had good mechanical properties and thermal stability, which was a safe and effective material for the treatment of carotid artery stenosis. In addition, the perioperative nursing method was effective and was of certain clinical adoption value.
Rationale:Primary biliary cholangitis (PBC) is a liver autoimmune disease. If this disease is associated with other liver injury factors, both misdiagnosis and missed diagnosis will easily occur. Therefore, detailed disease history collection and related laboratory examination should be performed on patients with liver injury for unidentified causes. When necessary, liver biopsy should be performed to confirm the histopathological diagnosis.Patient concerns:The subject patient was a 63-year-old Chinese male with chronic liver injury who had a drinking history of about 30 years and drank 500 g daily on average and began to take health products and dietary supplements (multivitamins) since June 2014.Diagnoses:Drug-induced liver injury (DILI) and alcoholic fatty liver disease (AFLD) were initially considered because the patient had a history of using health products (HP) and dietary supplements (DS) and drinking alcohol. However, he was subsequently considered with PBC based on the findings of anti-mitochondrial antibody positivity and elevated immunoglobulin level. Obstructive jaundice and space-occupying lesion in the liver were excluded by imaging examinations. Liver biopsy was performed to confirm the reasons for liver injury. Histopathological examination was conducted, and the patient was diagnosed with PBC associated with DILI and alcoholic liver fibrosis.Interventions:Ursodeoxycholic acid, glycyrrhizic acid, and methylprednisolone (small dose) were used to treat the patient.Outcomes:After 2 months, the serum levels of ALT, AST, AKP, GGT, and globulin returned to normal. After 4 months, the patient showed liver injury once again (an increase in ALT, AST, AKP, GGT and GLB) caused by repaglinide administration due to hyperglycemia. Ursodeoxycholic acid and methylprednisolone replaced the repaglinide administration. After 3 weeks, the levels of ALT, AST, AKP, GGT, and GLB returned to normal again.Lessons:The correct knowledge on PBC and early-stage recognition and diagnosis should be emphasized. When other causes of the liver injury cannot be excluded, liver biopsy is suggested. Histopathological change can be used to further clarify the reasons for liver injury and the principal contradiction as well as to guide the theraputic regimen.
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