Wastewater-based epidemiology has been used as a tool for surveillance of COVID-19 infections. This approach is dependent on the detection and quantification of SARS-CoV-2 RNA in untreated/raw wastewater. However, the quantification of the viral RNA could be influenced by the physico-chemical properties of the wastewater. This study presents the first use of Adaptive Neuro-Fuzzy Inference System (ANFIS) to determine the potential impact of physico-chemical characteristics of wastewater on the detection and concentration of SARS-CoV-2 RNA in wastewater. Raw wastewater samples from four wastewater treatment plants were investigated over four months. The physico-chemical characteristics of the raw wastewater was recorded, and the SARS-CoV-2 RNA concentration determined via amplification with droplet digital polymerase chain reaction. The wastewater characteristics considered were chemical oxygen demand, flow rate, ammonia, pH, permanganate value, and total solids. The mean SARS-CoV-2 RNA concentrations ranged from 648.1(±514.6) copies/mL to 1441.0(±1977.8) copies/mL. Among the parameters assessed using the ANFIS model, ammonia and pH showed significant association with the concentration of SARS-CoV-2 RNA measured. Increasing ammonia concentration was associated with increasing viral RNA concentration and pH between 7.1 and 7.4 were associated with the highest SARS-CoV-2 concentration. Other parameters, such as total solids, were also observed to influence the viral RNA concentration, however, this observation was not consistent across all the wastewater treatment plants. The results from this study indicate the importance of incorporating wastewater characteristic assessment into wastewater-based epidemiology for a robust and accurate COVID-19 surveillance.
Modeling surface water quality using soft computing techniques is essential for the effective management of scarce water resources and environmental protection. The development of accurate predictive models with significant input parameters and inconsistent datasets is still a challenge. Therefore, further research is needed to improve the performance of the predictive models. This study presents a methodology for dataset pre-processing and input optimization for reducing the modeling complexity. The objective of this study was achieved by employing a two-sided detection approach for outlier removal and an exhaustive search method for selecting essential modeling inputs. Thereafter, the adaptive neuro-fuzzy inference system (ANFIS) was applied for modeling electrical conductivity (EC) and total dissolved solids (TDS) in the upper Indus River. A larger dataset of a 30-year historical period, measured monthly, was utilized in the modeling process. The prediction capacity of the developed models was estimated by statistical assessment indicators. Moreover, the 10-fold cross-validation method was carried out to address the modeling overfitting issue. The results of the input optimization indicate that Ca2+, Na+, and Cl− are the most relevant inputs to be used for EC. Meanwhile, Mg2+, HCO3−, and SO42− were selected to model TDS levels. The optimum ANFIS models for the EC and TDS data showed R values of 0.91 and 0.92, and the root mean squared error (RMSE) results of 30.6 µS/cm and 16.7 ppm, respectively. The optimum ANFIS structure comprises a hybrid training algorithm with 27 fuzzy rules of triangular fuzzy membership functions for EC and a Gaussian curve for TDS modeling, respectively. Evidently, the outcome of the present study reveals that the ANFIS modeling, aided with data pre-processing and input optimization, is a suitable technique for simulating the quality of surface water. It could be an effective approach in minimizing modeling complexity and elaborating proper management and mitigation measures.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.