The search for technologies for crop improvement has been a continuous practice to address the food insecurity to the growing human population with an ever decreasing arable land and dynamic climate change around the world. Considering the potential technologies for crop improvement could close the rooms of poverty in developing countries in particular and around the globe at large. This review aimed to assess the site-directed mutation creation methods and to show the potential tools for future crop improvement programs. Site-directed mutagenesis was found to be an efficient process to create targeted mutation on cereal crops, horticultural crops, oilseed crops, and others. Agronomic traits such as yield, quality, and .
The search for technologies for crop improvement has been a continuous practice to address the food insecurity to the growing human population with an ever decreasing arable land and dynamic climate change around the world. Considering the potential technologies for crop improvement could close the rooms of poverty in developing countries in particular and around the globe at large. This review aimed to assess the site-directed mutation creation methods and to show the potential tools for future crop improvement programs. Site-directed mutagenesis was found to be an efficient process to create targeted mutation on cereal crops, horticultural crops, oilseed crops, and others. Agronomic traits such as yield, quality, and stress tolerance have been improved using site-directed mutagenesis. Besides, selectable marker elimination was also reported from transgenic crops by targeted mutation. Most of the reports on site-directed mutagenesis is focusing on cereal crops (58.339%) followed by horticultural crops (22.92%). Among the four mutagenic tools that have been reported, the CRISPR/Ca9 technology was found to be frequently used (66.67%) followed by TALENs. This tool is potential since it is efficient in creating targeted mutagenesis and less likely off-target effect, so it is repeatedly used in different research works. TALENs were used usually to knockout genes with bad traits. Moreover, the mutation created by mutagenic tools found to be efficient, and the mutated traits proved as it was heritable to generations. Hence, site-directed mutagenesis by the CRISPR/Cas9 system is advisable for agricultural development thereby ensuring food sustainability around the world.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.