BACKGROUND: Esophageal squamous cell carcinomas (ESCCs) tend to have multiple primary lesions, and it is believed that they arise from background mucosae with accumulation of genetic/epigenetic alterations. In this study, the objective was to elucidate the effects of smoking and drinking on the accumulation of epigenetic alterations in background mucosae. METHODS: Genes that are silenced in human ESCCs were searched for by treating 3 ESCC cell lines with the demethylating agent, 5‐aza‐2′‐deoxycytidine and performing oligonucleotide microarrays. Methylation levels were analyzed by quantitative methylation‐specific polymerase chain reaction analysis of 60 ESCCs and their corresponding background mucosae. RESULTS: Forty‐seven genes were identified as methylation‐silenced in at least 1 of the 3 ESCC cell lines, and 14 of those genes (claudin 6 [CLDN6]; G protein‐coupled receptor 158 [GPR158]; homeobox A9 [HOXA9]; metallothionein 1M [MT1M]; neurofilament, heavy polypeptide 200 kDa [NEFH]; plakophilin 1 [PKP1]; protein phosphatase 1, regulatory [inhibitor] subunit 14A [PPP1R14A]; pyrin domain and caspase recruitment domain containing [PYCARD]; R‐spondin family, member 4 [RSPO4]; testis‐specific protein, Y‐encoded–like 5 [TSPYL5]; ubiquitin carboxyl‐terminal esterase L1 [UCHL1]; zinc‐finger protein 42 homolog [ZFP42]; zinc‐finger protein interacting with K protein 1 homolog [ZIK1]; and zinc‐finger and SCAN domain containing 18 [ZSCAN18]) were used as markers. In the background mucosae, methylation levels of 5 genes (HOXA9, MT1M, NEFH, RSPO4, and UCHL1) had significant correlations with smoking duration (ρ = .268; P = .044; ρ = .405; P = .002; ρ = .285; P = .032; ρ = .300; P = .024; and ρ = .437; P = .001, respectively). In contrast, an inverse correlation between PYCARD methylation levels and alcohol intake was observed (ρ = −.334, P = .025) among individuals with the inactive aldehyde dehydrogenase 2 (ALDH2) genotype. CONCLUSIONS: The current results suggested that ESCCs developed from an epigenetic field for cancerization, which was induced by exposure to carcinogenic factors, such as tobacco smoking. The epigenetic field defect will be a novel target for risk diagnosis and prevention of ESCCs. Cancer 2009. © 2009 American Cancer Society.

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Abstract:Venezuela project and Australia project are recent typical overseas operator projects of Teikoku Oil Co.. Venezuela project is an on shore reactivation and development project, and Australia project is an offshore exploration project. Operational environments are fairly different. Each project selected own way of operation in order to achieve cost effective drilling operation.