Three colonial variants of Actinobacillus actinomycetemcomitans, which formed transparent rough (TR)-, transparent smooth (TS)-, and opaque smooth (OS)-surfaced colonies, were described in relation to their fimbriation. TR- and TS-cells were adhesive to agar and glass surfaces but not the OS-cells. The examination by electron microscopy revealed that TR-cells were highly fimbriated but not TS- and OS-cells. Thus, TS-cells seemed to be an intermediate type. The fimbriae were isolated from TR-cells by suspending in 0.15 M ethanolamine-HCl buffer (pH 10.5) and purified by dissolving non-fimbrial components in 0.5% deoxycholate and 0.7% n-octyl-beta-D-glucopyranoside. The relative molecular mass of the fimbrial subunit protein was 54,000.
Cucumber mosaic virus (CMV) and zucchini yellow mosaic virus (ZYMV) simultaneously inoculated on cucumber plants caused severer symptoms than those produced by CMV single infection, whilst weakened the symptoms caused by ZYMV. Determination of CMV concen tration by infectivity assay indicated that concentration of CMV in doubly infected plants was higher than those in singly infected plants. On the contrary, ZYMV concentration significantly decreased after mixed infection as determined by enzyme-linked immunosorbent assay. Yields of CMV obtained by sucrose density gradient centrifugation from doubly in fected cucumber plants were distinctly higher than those from singly infected ones about 3 weeks after inoculation.The enhanced multiplication of CMV was also found in doubly infected plants whether CMV was inoculated 1 or 2 weeks before or after ZYMV inocula tion. Electron microscopy of doubly infected leaf cells revealed abundant masses of CMV aggregates in the cytoplasm. Examination of CMV infected protoplasts prepared from sin gly and doubly infected cucumber leaves suggested that higher concentration of CMV in dou ble infection was due not only to the increase of CMV quantity in individual cells, but also to the increase in proportion of CMV infected cells.
Three colonial variants of Actinobacillus actinomycetemcomitans, which formed transparent rough (TR)‐, transparent smooth (TS)‐, and opaque smooth (OS)‐surfaced colonies, were described in relation to their fimbriation. TR‐ and TS‐cells were adhesive to agar and glass surfaces but not the OS‐cells. The examination by electron microscopy revealed that TR‐cells were highly fimbriated but not TS‐ and OS‐cells. Thus, TS‐cells seemed to be an intermediate type. The fimbriae were isolated from TR‐cells by suspending in 0.15 M ethanolamine‐HCl buffer (pH 10.5) and purified by dissolving non‐fimbrial components in 0.5% deoxycholate and 0.7% n‐octyl‐β‐d‐glucopyranoside. The relative molecular mass of the fimbrial subunit protein was 54 000.
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