The objective of this research was to evaluate the effect of H 3 PO 4 concentrations and particle size of eggshell used in the feed of laying hens on bone and blood profiles. Ninety-six laying hens (Isa Brown strain) age 25 weeks were reared in individual battery cage and divided into 8 groups randomly. Group 1 was fed using eggshell without H 3 PO 4 and particle size of <1 mm (feed 1 ), group 2 was fed using eggshell that has been soaked in H 3 PO 4 3% and particle size of <1 mm (feed 2), group 3 were fed using eggshell that has been soaked in H 3 PO 4 4% and particle size of <1 mm (feed 3), group 4 was fed using eggshell that has been soaked in H 3 PO 4 5% and particle size of <1 mm (feed 4), group 5 was fed using eggshell that without H 3 PO 4 and particle size of <3 mm (feed 5 ), group of 6 was fed using eggshell that has been soaked in H 3 PO 4 3% and particle size of <3 mm (feed 6), group 7 was fed using eggshell that has been soaked in H 3 PO 4 4 % and particle size of <3 mm (feed 7) and a group of 8 was fed using eggshell that has been soaked in H 3 PO 4 5% and particle size of <3 mm (feed 8). A Completely Randomized Design patterns factorial 4 x 2 x 3 was used in this research. Result of this research showed that had no interaction effect (P>0.05) between the H 3 PO 4 concentration and particle size of eggshell on weight, volume, diameter of tibia bone and calcium and phosphorus content of the blood. The concentration of H 3 PO 4 or particles size also had no effect (P>0.05) on all variables.
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